Transforming growth factor-beta improves healing of radiation-impaired wounds

Journal Article · Sun Sep 01 04:00:00 EDT 1991 · Journal of Investigative Dermatology; (United States)

DOI:https://doi.org/10.1111/1523-1747.ep12481258· OSTI ID:5262194

Bernstein, E F; Harisiadis, L; Salomon, G; Norton, J; Sollberg, S; Uitto, J; Glatstein, E; Glass, J; Talbot, T; Russo, A ^[1]

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1. Radiation Oncology Branch, National Institutes of Health, Bethesda, MD (USA)

Exogenously applied TGF-{beta} 1 has been shown to increase wound strength in incisional wounds early in the healing process. An impaired wound healing model was first established in guinea pigs by isolating flaps of skin and irradiating the flaps to 15 Gray in one fraction using a 4-MeV linear accelerator. Incisions made 2 d after irradiation were excised 7 d later, and showed decreased linear wound bursting strength (WBS) as compared to non-irradiated control wounds on the contralateral side of each animal (p = 0.001). The effect of TGF-{beta}on healing of radiation-impaired wounds was studied using this model. Skin on both left and right sides of guinea pigs was irradiated as above. A linear incision was made in each side. Collagen with either 1, 5, or 20 micrograms of TGF-{beta} was applied to one side prior to closure with staples, whereas the contralateral side received saline in collagen. Wounds given either 1 or 5 micrograms of TGF-{beta} were found to be stronger than controls at 7 d (p less than 0.05), whereas those receiving the higher 20-micrograms dose were weaker than controls (p less than 0.05). Thus, TGF-{beta} in lower doses improved healing at 7 d but very large amounts of the growth factor actually impaired healing. In situ hybridization done on wound samples showed increased type I collagen gene expression by fibroblasts in wounds treated with 1 micrograms TGF-{beta} over control wounds. These results indicate that TGF-{beta} improved wound healing as demonstrated by increased WBS. This improvement is accompanied by an up-regulation of collagen gene expression by resident fibroblasts.

• OSTI ID: 5262194
• Journal Information: Journal of Investigative Dermatology; (United States), Journal Name: Journal of Investigative Dermatology; (United States) Vol. 97:3; ISSN 0022-202X; ISSN JIDEA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
COLLAGEN
CONNECTIVE TISSUE CELLS
DOSES
FIBROBLASTS
GENE REGULATION
GROWTH FACTORS
GUINEA PIGS
HEALING
INJURIES
MAMMALS
MITOGENS
ORGANIC COMPOUNDS
PROTEINS
RADIATION DOSES
RADIATION EFFECTS
RADIATION INJURIES
RADIOSENSITIVITY EFFECTS
RECOVERY
RODENTS
SCLEROPROTEINS
SOMATIC CELLS
VERTEBRATES
WOUNDS