Impact of ozone on the activity and quantity of ribulose bisphosphate carboxylase/oxygenase in potato foliage and its relation to premature senescence

Thesis/Dissertation · Fri Jan 01 04:00:00 EST 1988

OSTI ID:5257016

Dann, M S

Plants, 26 days old, were exposed to O{sub 3} for five days in a controlled environment chamber. Initial and total activities and quantity of enzyme declined in both O{sub 3}-treated and control plants. Ozone accelerated the decline and produced a significantly greater decrease in activity and quantity by the fifth day of O{sub 3} exposure. Percent activation of the enzyme did not change. Enzyme activity and quantity of O{sub 3}-treated plants remained below control levels throughout a seven-day post-exposure observation period. Ozone may accelerate the decline in rubisco by increasing enzyme acceptability to proteases. To test this hypothesis, rubisco was purified from Norland potato foliage and the enzyme exposed to O{sub 3} or oxygen (O{sub 2}) in vitro. Following oxidant treatment, the extract was treated with exogenous protease. Oxidant treatment reduced the quantity of rubisco an average of 15%. Addition of protease further reduced the quantity by another 30% after O{sub 3} but no O{sub 2} treatment.

• Research Organization: Pennsylvania State Univ., University Park, PA (USA)
• OSTI ID: 5257016
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CARBON-CARBON LYASES
CARBOXY-LYASES
CELL DIFFERENTIATION
ENZYME ACTIVITY
ENZYMES
EXPOSURE CHAMBERS
LEAVES
LYASES
MAGNOLIOPHYTA
MAGNOLIOPSIDA
OXIDOREDUCTASES
OXYGENASES
OZONE
PLANTS
RIBULOSE DIPHOSPHATE CARBOXYLASE
SENSITIVITY
SOLANUM
SOLANUM TUBEROSUM
TOXICITY