Bioremediation of soil and ground water impacted with organic contaminants

Conference · Fri Feb 01 04:00:00 EST 1991 · AAPG Bulletin (American Association of Petroleum Geologists); (United States)

OSTI ID:5253202

Woods, W B ^[1]

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1. EMCON Associates, San Jose, CA (United States)

Two case studies demonstrate the controlled use of micro-organisms to degrade organic contaminants under aerobic and anaerobic conditions. The aerobic study illustrates the degradation of hydrocarbons in a soil matrix. Data are presented that show a two-phase degradation of total petroleum hydrocarbons (TPH) from about 1,300 ppm TPH to cleanup levels of 100 ppm or less in two months. Total aerobic microorganism and substate-specific degrader counts were tracked throughout the study. Typical total aerobic counts of 10{sup 6} colony forming units (CFU)/g and hydrocarbon degrader counts of 10{sup 4} CFU/g were observed. Hydrocarbon degraders were enumerated on minimal salts media incubated in the presence of hydrocarbon vapors. The anaerobic study documents the successful use of a supplemental carbon source and fertilizers to stimulate indigenous microbe to degrade ketones. A nutrient mix of s polysaccharide, a nitrate electron acceptor and an inorganic orthophosphate was used to augment 100,000 yd{sup 3} of soil contaminated with ketones at about 1,000 ppm. The key elements of a biotreatment project are discussed (i.e., site characterization, treatability studies, biotreatment design, site construction, system maintenance, final disposal and site closure). Lastly, the benefits of bioremediation vs. other remediation alternatives such as landfill disposal, incineration, and stabilization/fixation are discussed in terms of cost and liability.

• OSTI ID: 5253202
• Report Number(s): CONF-9103128--
• Conference Information: Journal Name: AAPG Bulletin (American Association of Petroleum Geologists); (United States) Journal Volume: 75:2
• Country of Publication: United States
• Language: English

Related Subjects

02 PETROLEUM
020900* -- Petroleum-- Environmental Aspects
54 ENVIRONMENTAL SCIENCES
540250 -- Environment
Terrestrial-- Site Resource & Use Studies-- (1990-)
BIODEGRADATION
CARBOHYDRATES
CHEMICAL REACTIONS
CLEANING
COMPARATIVE EVALUATIONS
CONTROL
COST BENEFIT ANALYSIS
DECOMPOSITION
DECONTAMINATION
ECOLOGICAL CONCENTRATION
ECONOMIC ANALYSIS
ECONOMICS
ENERGY SOURCES
EVALUATION
FOSSIL FUELS
FUELS
GROUND WATER
HYDROGEN COMPOUNDS
KETONES
LAND POLLUTION
LAND POLLUTION CONTROL
MICROORGANISMS
OIL SPILLS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PETROLEUM
POLLUTION
POLLUTION CONTROL
POLYSACCHARIDES
REMOVAL
SACCHARIDES
SOILS
WATER
WATER POLLUTION
WATER POLLUTION CONTROL