Excimer laser desorption mass spectrometry of biomolecules at 248 and 193 nm

Journal Article · Wed Dec 16 23:00:00 EST 1987 · J. Phys. Chem.; (United States)

DOI:https://doi.org/10.1021/j100310a016· OSTI ID:5248773

Spengler, B; Karas, M; Bahr, U; Hillenkamp, F

Laser desorption mass spectrometry (LD-MS) of various peptides has been performed with excimer laser nanosecond pulses of 248- and 193-nm wavelengths. Whereas the spectra obtained with 248 nm resemble those obtained earlier with quadrupled Nd:YAG lasers at 266 nm, the peptides show a quite different fragmentation and association behavior at 193 nm. These observations can help in understanding the desorption and ion formation process. They indicate a wavelength-dependent competition between photochemical ion formation and ion generation via a collective process of the strongly perturbed solid. They can also help to further optimize the analysis of given organic molecules by LD-MS.

• Research Organization: Universitaet Muenster (West Germany)
• OSTI ID: 5248773
• Journal Information: J. Phys. Chem.; (United States), Journal Name: J. Phys. Chem.; (United States) Vol. 91:26; ISSN JPCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400500* -- Photochemistry
DATA
DESORPTION
ELECTROMAGNETIC RADIATION
EXCIMER LASERS
EXPERIMENTAL DATA
GAS LASERS
INFORMATION
IONIZATION
LASERS
MASS SPECTROSCOPY
MEASURING INSTRUMENTS
NUMERICAL DATA
ORGANIC COMPOUNDS
PEPTIDES
PHOTOIONIZATION
PROTEINS
RADIATIONS
SPECTROMETERS
SPECTROSCOPY
TIME-OF-FLIGHT SPECTROMETERS
ULTRAVIOLET RADIATION
VISIBLE RADIATION