Ganglioside GM/sub 1/ spontaneous transfer between phospholipid vesicles

Conference · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5246635

Brown, R E; Sugar, I P; Thompson, T E

The transfer kinetics of the monosiaylated glycosphingolipid, GM/sub 1/, between different size phospholipid vesicles was measured using molecular sieve chromatography. At desired time intervals, small unilamellar donor vesicles were separated from large unilamellar acceptor vesicles by elution from a Sephacryl S-500 column (/sup 3/H)-GM/sub 1/ net transfer was calculated relative to (/sup 14/C)-cholesteryl oleate, which served as a nontransferable marker in the donor vesicles. The initial GM/sub 1/ transfer rate between 1-palmitoyl-2-oleoyl phosphatidylcholine vesicles at 45/sup 0/C deviated slightly from first order kinetics and possessed a half time of 3.6 days. This transfer half time is an order of magnitude shorter than that observed from the desiaylated derivative of GM/sub 1/. The transfer kinetics are consistent with the authors recent electron microscopic results suggesting a molecular distribution of GM/sub 1/ in liquid-crystalline phosphatidylcholine bilayers.

• Research Organization: Univ. of Virginia Medical School, Charlottsville
• OSTI ID: 5246635
• Report Number(s): CONF-8606151-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL HALF-LIFE
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
ESTERS
GANGLIOSIDES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIPIDS
MONOCARBOXYLIC ACIDS
OLEIC ACID
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
REACTION KINETICS
SACCHARIDES
SEPARATION PROCESSES
TRACER TECHNIQUES
TRITIUM COMPOUNDS