Structural analysis of N-linked oligosaccharides from glycoproteins secreted by Dictyostelium discoideum: identification of mannose 6-sulfate
The N-linked oligosaccharides found on the lysosomal enzymes from Dictyostelium discoideum are highly sulfated and contain methylphosphomannosyl residues. Here the authors report studies done on the structure of N-linked oligosaccharides found on proteins secreted during growth, a major portion of which are lysosomal enzymes. Cells were metabolically labeled with (2-/sup 3/H)Man and /sup 35/SO/sub 4/ and a portion of the oligosaccharides were released by a sequential digestion with endoglycosidase H followed by endoglycosidase/peptide N-glycosidase F preparations. The oligosaccharides were separated by anion exchange high performance liquid chromatography into fractions containing from one up to six negative charges. Some of the oligosaccharides contained only sulfate esters or phosphodiesters, but most contained both. Less than 2% of the oligosaccharides contained a phosphomonoester or an acid-sensitive phosphodiester typical of the mammalian lysosomal enzymes. A combination of acid and base hydrolysis suggested that most of the sulfate esters were linked to primary hydroxyl groups. The presence of Man-6-SO/sub 4/ was demonstrated by the appearance of 3,6-anhydromannose in acid hydrolysates of base-treated, reduced oligosaccharides.
- Research Organization:
- Univ. of California, San Diego, La Jolla
- OSTI ID:
- 5246503
- Journal Information:
- J. Biol. Chem.; (United States), Vol. 261:1
- Country of Publication:
- United States
- Language:
- English
Similar Records
Interaction of Dictyostelium discoideum lysosomal enzymes with the mammalian phosphomannosyl receptor. The importance of oligosaccharides which contain phosphodiesters
Dictyostelium discoideum: mutants in the biosynthesis of the lipid-linked precursor of N-linked oligosaccharides
Related Subjects
FUNGI
OLIGOSACCHARIDES
STRUCTURAL CHEMICAL ANALYSIS
ENZYMES
EXPERIMENTAL DATA
GLUCOPROTEINS
GROWTH
HYDROLYSIS
ION EXCHANGE
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
LYSOSOMES
MANNOSE
PHOSPHORIC ACID ESTERS
SULFUR 35
SULFURIC ACID ESTERS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALDEHYDES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DATA
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
ESTERS
EVEN-ODD NUCLEI
HEXOSES
INFORMATION
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LYSIS
MONOSACCHARIDES
NUCLEI
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANOIDS
PLANTS
PROTEINS
RADIOISOTOPES
SACCHARIDES
SEPARATION PROCESSES
SOLVOLYSIS
SULFUR ISOTOPES
550201* - Biochemistry- Tracer Techniques