Synthesis of peptide fragments of the hemagglutinin of influenza virus A/Aichi/2/68 (H/sub 3/N/sub 2/)
Peptides corresponding to sections 122-133, 136-147, and 154-164 of the heavy chain of the hemagglutinin of influenza virus A/Aichi/2/68 (H/sub 3/N/sub 2/) have been synthesized. The synthesis was performed by the stepwise growth of the peptide chain by means of activated esters of Boc-amino acids and the condensation of peptide blocks by the carbodiimide method in solution. The colored 2-(4-(phenylazo)benylsulfonyl)ethyl group, which can be removed by bases, was used for the protection of the C-terminal carboxy groups of the peptides and peptide blocks, facilitating the purification of the peptides synthesized. After the elimination of the N- and C-terminal carboxy groups of the peptides and peptide blocks, facilitating the purification of the peptides synthesized. After the elimination of the N- and C-terminal protective groups, the peptides were deblocked with 1 M trifluoromethanesulfonic acid in trifluoroacetic acid containing 10% of thioanisole and were purified by prepatative reversed-phase chromatography. Conjugates of the peptides synthesized with bovine serum albumin have been obtained.
- Research Organization:
- All-Union Scientific-Research Institute of Molecular Biology, Novosibirsk (USSR)
- OSTI ID:
- 5244049
- Journal Information:
- Sov. J. Bioorg. Chem. (Engl. Transl.); (United States), Vol. 11:8; Other Information: Translated from Bioorganicheskaya Khim.; 11: No. 8, 1037-1047(Aug 1985)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
HEMAGGLUTININS
AMINO ACID SEQUENCE
BIOLOGICAL EFFECTS
BIOSYNTHESIS
RADIOIMMUNOASSAY
PEPTIDES
ACETIC ACID
ACYLATION
AMINO ACIDS
ANTIGENS
CARBON 14 COMPOUNDS
CARBOXYLIC ACID ESTERS
DEHYDROCYCLIZATION
INFLUENZA VIRUSES
LABELLING
ORGANIC FLUORINE COMPOUNDS
SULFONIC ACID ESTERS
TRITIUM COMPOUNDS
VACCINES
AGGLUTININS
ANTIBODIES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
ESTERS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MICROORGANISMS
MOLECULAR STRUCTURE
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASITES
PROTEINS
RADIOASSAY
RADIOIMMUNOLOGY
SYNTHESIS
TRACER TECHNIQUES
VIRUSES
550201* - Biochemistry- Tracer Techniques