Comparative immunohistologic studies in an adoptive transfer model of acute rat cardiac allograft rejection

Journal Article · Mon Jul 01 00:00:00 EDT 1985 · Transplantation; (United States)

OSTI ID:5243818

Forbes, R D; Lowry, R P; Gomersall, M; Blackburn, J

It has been shown that fulminant acute rejection of rat cardiac allografts across a full haplotype disparity may occur as a direct result of adoptive transfer of sensitized W3/25+ MRC OX8- SIg- T helper/DTH syngeneic spleen cells to sublethally irradiated recipients. In order to establish the immunohistologic parameters of this form of rejection, allografts and recipient lymphoid tissue were analyzed using a panel of monoclonal antibodies of known cellular distribution. These data were compared with those obtained following reconstitution of irradiated allograft recipients with unseparated sensitized spleen cells, with unreconstituted irradiated donor recipient pairs, with unmodified first-set rejection, and with induced myocardial infarction of syngeneic heart grafts transplanted to normal and to sublethally irradiated recipients. Rejecting cardiac allografts transplanted to all reconstituted irradiated recipients were characterized by extensive infiltration with MRC OX8+ (T cytotoxic-suppressor, natural killer) cells even when this subset was virtually excluded from the reconstituting inocula. A similar proportional accumulation of MRC OX8+ cells observed at the infarct margins of syngeneic heart grafts transplanted to irradiated unreconstituted recipients greatly exceeded that present in normal nonirradiated controls. These data provide evidence that under conditions of heavy recipient irradiation, MRC OX8+ cells may be sequestered within heart grafts in response to nonspecific injury unrelated to the rejection process.

• Research Organization: Royal Victoria Hospital, Montreal, Quebec, Canada
• OSTI ID: 5243818
• Journal Information: Transplantation; (United States), Vol. 1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
HEART
TRANSPLANTS
RATS
GRAFT-HOST REACTION
BIOLOGICAL MODELS
COMPARATIVE EVALUATIONS
LEUKOCYTES
LYMPH NODES
LYMPHOCYTES
MONOCLONAL ANTIBODIES
MYOCARDIAL INFARCTION
RADIATION CHIMERAS
SPLEEN CELLS
SUBLETHAL IRRADIATION
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CHIMERAS
CONNECTIVE TISSUE CELLS
DISEASES
IRRADIATION
LYMPHATIC SYSTEM
MAMMALS
MATERIALS
MOSAICISM
ORGANS
RODENTS
SOMATIC CELLS
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques
560152 - Radiation Effects on Animals- Animals