Binding of apolipoprotein A-I and apolipoprotein A-IV to cultured bovine aortic endothelial cells

Journal Article · Tue Mar 01 04:00:00 EST 1988 · Arteriosclerosis (Dallas); (United States)

DOI:https://doi.org/10.1161/01.ATV.8.2.178· OSTI ID:5243783

Savion, N; Gamliel, A

Adult bovine aortic endothelial (ABAE) cells specifically bound /sup 125/I-labelled high density lipoprotein3 (/sup 125/I-HDL3), and saturation of the binding was observed at a concentration of about 50 to 100 micrograms protein/ml. The binding of purified apolipoproteins (apo) A-I and A-IV to cultured ABAE cells and the correlation of this binding with the binding of HDL to the cells was studied. ABAE cells bound /sup 125/I-labelled apo A-I (/sup 125/I-apo A-I) and after a 3-hour exposure to the ligand, most of the radioactivity (82%) was associated with the cell surface and was accessible for trypsinization. Saturation of /sup 125/I-apo A-I binding was observed at a concentration of 3 micrograms/ml; each cell possessed 1.38 X 10(5) high affinity binding sites (Kd = 2.04 X 10(-8) M). The cultures also bound /sup 125/I-labelled apo A-IV (/sup 125/I-apo A-IV), and saturation of the binding was observed at a concentration of 2 micrograms/ml. Each cell possessed 5.4 X 10(4) high affinity binding sites for apo A-IV (Kd = 1.45 X 10(-8) M). Addition of either excess unlabelled apo A-I or excess unlabelled apo A-IV competed with the binding of both iodinated apo A-I and apo A-IV. It suggests that apo A-I and A-IV share the same binding site on endothelial cells. HDL also successfully competed with the binding of apo A-I and A-IV to ABAE cells.

• Research Organization: Sheba Medical Center, Tel Hashomer (Israel)
• OSTI ID: 5243783
• Journal Information: Arteriosclerosis (Dallas); (United States), Journal Name: Arteriosclerosis (Dallas); (United States) Vol. 8:2; ISSN ARTRD
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ANIMAL TISSUES
ANIMALS
AORTA
APOLIPOPROTEINS
ARTERIES
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
CATTLE
CELL CULTURES
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGANDS
LIPIDS
LIPOPROTEINS
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RUMINANTS
TISSUES
TRACER TECHNIQUES
VERTEBRATES