Effect of captan on the exonuclease activities of DNA polymerase I from E. coli and reverse transcriptase from avian myeloblastosis virus
Thesis/Dissertation
·
OSTI ID:5243716
The DNA pol I polymerase activity is known to be inhibited by captan. When captan was tested for its ability to alter the exonuclease activity of DNA pol I, degradation was enhanced at high substrate concentrations. At low concentrations of DNA, captan was inhibitory. By assaying the two exonuclease activities separately it was shown that the differential effect by captan was the result of a combined inhibition of the 3' ..-->.. 5' exonuclease and enhancement of the 5' ..-->.. 3' exonuclease. Studies employing (/sup 14/C) captan showed that the alterations in DNA pol I activities were a result of the irreversible binding of captan to the enzyme in a ratio of 1:1. The effect of captan on AMV reverse transcriptase RNase H activity was also studied. RNase H activity appeared to be more sensitive to captan than was the polymerase activity. Inhibition of the polymerase activity could be prevented by deoxynucleotide triphosphate and was increased by templateprimer. RNase H activity, which showed a sigmoidal relationship between activity and substrate concentration, decreased in V/sub max/ with no change in the Hill coefficient in the presence of captan
- Research Organization:
- Nevada Univ., Reno (USA)
- OSTI ID:
- 5243716
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIRDS
CARBON 14 COMPOUNDS
DNA POLYMERASES
ENZYME ACTIVITY
ENZYMES
ESCHERICHIA COLI
ESTERASES
FUNGICIDES
HYDROLASES
INHIBITION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MICROORGANISMS
NUCLEOTIDYLTRANSFERASES
PARASITES
PESTICIDES
PHOSPHODIESTERASES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
REACTION KINETICS
RNA-ASE
TRACER TECHNIQUES
TRANSFERASES
VERTEBRATES
VIRUSES
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIRDS
CARBON 14 COMPOUNDS
DNA POLYMERASES
ENZYME ACTIVITY
ENZYMES
ESCHERICHIA COLI
ESTERASES
FUNGICIDES
HYDROLASES
INHIBITION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MICROORGANISMS
NUCLEOTIDYLTRANSFERASES
PARASITES
PESTICIDES
PHOSPHODIESTERASES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
REACTION KINETICS
RNA-ASE
TRACER TECHNIQUES
TRANSFERASES
VERTEBRATES
VIRUSES