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Autoradiographic visualization of muscarinic receptors in human bronchi

Journal Article · · J. Pharmacol. Exp. Ther.; (United States)
OSTI ID:5243651
; ; ; ; ;
To visualize muscarinic receptors in human bronchi, the stripping film method was used which permits direct autoradiographic localization of tissue labeling. Cryostate sections of human bronchi were fixed in 0.5% glutaraldehyde in Krebs-Ringer buffer, pH 7.0 for 30 min at 0/sup 0/C, washed in Krebs-Ringer buffer for 20 min at 0/sup 0/C and incubated with (-)-(/sup 3/H)Quinuclidinyl benzilate ((-)-(/sup 3/H)QNB) for 90 min at 37/sup 0/C. Specific (-)-(/sup 3/H)QNB binding to tissue sections was saturable (receptor density of 0.14 +/- 0.03 fmol/tissue section) and of high affinity (Kd of 40 +/- 9 pM). For autoradiography, labeled tissue sections were covered with stripping film and exposed for 5 months. Muscarinic receptors in human bronchi were located predominantly in submucosal glands and parasympathetic ganglia. There was less labeling in smooth muscle cells and nerve bundles. Epithelium and blood vessels located within the bronchial wall were devoid of specific labeling.
Research Organization:
Univ. of Nijmegen (Netherlands)
OSTI ID:
5243651
Journal Information:
J. Pharmacol. Exp. Ther.; (United States), Journal Name: J. Pharmacol. Exp. Ther.; (United States) Vol. 244:2; ISSN JPETA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL LOCALIZATION
BLOOD VESSELS
BODY
BRONCHI
CARDIOVASCULAR SYSTEM
DOSE-RESPONSE RELATIONSHIPS
DRUGS
EPITHELIUM
KINETICS
LABELLED COMPOUNDS
MAMMALS
MAN
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
ORGANS
PARASYMPATHOMIMETICS
PRIMATES
PROTEINS
REACTION KINETICS
RECEPTORS
RESPIRATORY SYSTEM
TISSUES
TRITIUM COMPOUNDS
VERTEBRATES