Phosphorus-31 nuclear magnetic resonance studies of cellular systems

Thesis/Dissertation · Wed Jan 01 04:00:00 EST 1986

OSTI ID:5243530

Robitaille, P M.L.

In this study, /sup 31/P-NMR spectroscopy was applied to the study of (1) sipunculan erythrocytes, (2) spermatozoa isolated from several vertebrate and invertebrate species, and (3) unfertilized eggs isolated from the blue crab and the horseshoe crab. /sup 31/P-NMR results center on the identification of key metabolites and on the determination of intracellular pH. In studies involving fish spermatozoa, emphasis was also placed on examining changes in metabolic profiles following (1) an anaerobic insult, (2) motility initiation, or (3) short-term storage. This study also captures several difficulties in spectral interpretation which a spectroscopist is likely to encounter.

• Research Organization: Iowa State Univ. of Science and Technology, Ames (USA)
• OSTI ID: 5243530
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AQUATIC ORGANISMS
ARTHROPODS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CRABS
CRUSTACEANS
DECAPODS
EGGS
ERYTHROCYTES
FISHES
GAMETES
GERM CELLS
INVERTEBRATES
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MATERIALS
METABOLITES
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-EVEN NUCLEI
PHOSPHORUS 31
PHOSPHORUS ISOTOPES
RESONANCE
SPECTRA
SPERMATOZOA
STABLE ISOTOPES
VERTEBRATES