UV cross-linking of the Bacillus subtilis RNA polymerase to DNA in promoter and non-promoter complexes
Complexes between Bacillus subtilus RNA polymerase and /sup 32/P-labeled DNA were irradiated with UV light and digested with nuclease; electrophoresis and autoradiography were used to identify the polymerase subunits cross-linked to DNA. These experiments showed: 1) that cross-linkage of promoter complexes yielded predominantly the beta and sigma subunits; 2) that beta, beta', and sigma were detected in non-promoter complexes; 3) that addition of the delta subunit or high concentrations of NaCl decreased cross-linkage of all subunits, especially the cross-linkage of the sigma subunit in non-promoter complexes and the binding of polymerase at DNA ends; 4) that different patterns of cross-linkage were obtained at 0 degrees C (conditions favoring the formation of closed complexes) and 37 degrees C (conditions favoring the formation of open complexes); and 5) predominantly beta and possibly alpha were cross-linked by irradiation of core-DNA complexes whereas similar experiments with core-delta complexed to DNA showed the efficient cross-linkage of beta' and beta.
- Research Organization:
- Univ. of Washington, Seattle
- OSTI ID:
- 5243416
- Journal Information:
- J. Biol. Chem.; (United States), Vol. 13
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
GENETIC RADIATION EFFECTS
RNA POLYMERASES
CROSS-LINKING
AUTORADIOGRAPHY
BACILLUS SUBTILIS
BACTERIOPHAGES
CHEMICAL BONDS
ELECTROPHORESIS
LABELLED COMPOUNDS
PHOSPHORUS 32
PLASMIDS
SODIUM CHLORIDES
TRACER TECHNIQUES
ULTRAVIOLET RADIATION
ALKALI METAL COMPOUNDS
BACILLUS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHLORIDES
CHLORINE COMPOUNDS
DAYS LIVING RADIOISOTOPES
ELECTROMAGNETIC RADIATION
ENZYMES
GENETIC EFFECTS
HALIDES
HALOGEN COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
POLYMERIZATION
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
SODIUM COMPOUNDS
TRANSFERASES
VIRUSES
560112* - Radiation Effects on Biochemicals- In Microorganisms- (-1987)