Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Modulation of muscarinic and micotinic cholinergic receptor mediated catecholamine secretion in guinea pig chromaffin cells by phorbol esters

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5243169
; ;

Isolated guinea pig chromaffin cells possess both nicotinic (nAChR) and muscarinic (mAChR) cholinergic receptors that are positively coupled to catecholamine (CA) release. Sixty to 70% of CA release is mediated by nAChRs and 30-40% by mAChRs. In the absence of added calcium, nAChR mediated CA release was reduced by 65% whereas the muscarinic response was unaffected. The addition of 100nM 12-0-tetradecanoylphorbol-13-acetate (TPA), an activator of protein kinase C (PKC), also resulted in an increased CA release. Temporally and quantitatively, this response resembled that of mAChR activation. Addition of optimal concentrations of nicotine (50..mu..M) and TPA (100nM) induced a synergistic increase in CA release. Addition of muscarine (1mM) and TPA resulted in an additive response despite a 40-60% inhibition of mAChR mediated inositol phosphate release by TPA. Thus, in guinea pig chromaffin cells, it appears that PKC activation alone is a sufficient stimulus for CA release and that activation of both nicotinic and muscarinic receptors may further increase this enzyme's activity.

Research Organization:
New York Medical College, Valhalla
OSTI ID:
5243169
Report Number(s):
CONF-8606151-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
Country of Publication:
United States
Language:
English

Similar Records

Mixed nicotinic and muscarinic features of cholinergic receptor coupled to secretion in bovine chromaffin cells
Journal Article · Sat Jun 01 00:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States) · OSTI ID:6096979
Selective inhibition of cholinergic receptor-mediated /sup 45/Ca++ uptake and catecholamine secretion from adrenal chromaffin cells by taxol and vinblastine
Journal Article · Mon Oct 01 00:00:00 EDT 1984 · J. Pharmacol. Exp. Ther.; (United States) · OSTI ID:5948032
Identification, characterization, and regulation of a nicotinic acetylcholine receptor on bovine adrenal chromaffin cells in culture
Thesis/Dissertation · Thu Dec 31 23:00:00 EST 1987 · OSTI ID:7018890

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALOIDS
AMINES
ANIMAL CELLS
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZINES
AZOLES
BIOLOGICAL EFFECTS
CARCINOGENS
CATECHOLAMINES
DRUGS
ENZYME ACTIVITY
ENZYMES
ESTERS
GUINEA PIGS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
NICOTINE
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASYMPATHOLYTICS
PARASYMPATHOMIMETICS
PHENOLS
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
POLYPHENOLS
PROTEINS
PYRIDINES
PYRROLES
PYRROLIDINES
RECEPTORS
RODENTS
SECRETION
SYMPATHOMIMETICS
SYNERGISM
TRANSFERASES
VERTEBRATES