Tumor promoter phorbol myristate acetate inhibits Ca/sup 2 +/ influx through voltage-gated Ca/sup 2 +/ channels in two secretory cell lines, PC12 and RINm5F
Protein kinase C is known to be involved both in initiation and termination of cellular responses due to phosphoinositide breakdown. The authors report that in PC12 cells (a line of neurosecretory cells derived from a rat pheochromocytoma, pretreatment with nanomolar concentrations of phorbol myristate acetate, PMA, which is believed to specifically activate protein kinase C, inhibits the cytosolic-free Ca/sup 2 +/ concentration rise induced by depolarizing agents. In contrast, plasma membrane potential and /sup 45/Ca efflux from preloaded cells were unaffected by PMA pretreatment. Inhibition by PMA and diacylglycerol of the cytosolic-free Ca/sup 2 +/ concentration rise induced by depolarization was observed also in another cell line, the insulin secreting line RINm5F. These results raise the possibility that the voltage-gated Ca/sup 2 +/ channel is under inhibitory control by protein kinase C.
- Research Organization:
- Univ. of Padova, Italy
- OSTI ID:
- 5243067
- Journal Information:
- J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 261:1; ISSN JBCHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOELECTRICITY
BIOLOGICAL EFFECTS
CALCIUM 45
CALCIUM COMPOUNDS
CALCIUM ISOTOPES
CARCINOGENS
CATIONS
CELL CONSTITUENTS
CELL MEMBRANES
CHARGED PARTICLES
CHEMICAL ACTIVATION
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRICITY
ENZYMES
ESTERS
EVEN-ODD NUCLEI
INHIBITION
INTERMEDIATE MASS NUCLEI
IONS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
MEMBRANE TRANSPORT
MEMBRANES
NUCLEI
ORGANIC COMPOUNDS
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PLASMA CELLS
PROMOTERS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SOMATIC CELLS
TRACER TECHNIQUES
TRANSFERASES
TUMOR PROMOTERS