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Human cDNA clones for an. cap alpha. subunit of G/sub i/ signal-transduction protein

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
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Two cDNA clones were obtained from a lambdagt11 cDNA human brain library that correspond to ..cap alpha../sub i/ subunits of G signal-transduction proteins (where ..cap alpha../sub i/ subunits refer to the ..cap alpha.. subunits of G proteins that inhibit adenylate cyclase). The nucleotide sequence of human brain ..cap alpha../sub i/ is highly homologous to that of bovine brain ..cap alpha../sub i/ and the predicted amino acid sequences are identical. However, human and bovine brain ..cap alpha../sub i/ cDNAs differ significantly from ..cap alpha../sub i/ cDNAs from human monocytes, rat glioma, and mouse macrophages in amino acid (88% homology) and nucleotide (71-75% homology) sequences. In addition, the nucleotide sequences of the 3' untranslated regions of human and bovine brain ..cap alpha../sub i/ cDNAs differ markedly from the sequences of human monocyte, rat glioma, and mouse macrophage ..cap alpha../sub i/ cDNAs. These results suggest there are at least two classes of ..cap alpha../sub i/ mRNA

Research Organization:
National Institutes of Health, Bethesda, MD (USA)
OSTI ID:
5242991
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:15; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
BRAIN
CATTLE
CENTRAL NERVOUS SYSTEM
CLONING
CYCLASES
DAYS LIVING RADIOISOTOPES
DNA
DNA-CLONING
DOMESTIC ANIMALS
ENZYMES
ISOTOPES
LIGHT NUCLEI
LYASES
MAMMALS
MEMBRANE PROTEINS
MESSENGER-RNA
MOLECULAR STRUCTURE
NERVOUS SYSTEM
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RECEPTORS
RECOMBINANT DNA
RNA
RUMINANTS
VERTEBRATES