Synthesis of 3'-azido and 3'-aminoarabinonucleoside 5'-triphosphates and study of their substrate properties in systems with polynucleotide-synthesizing enzymes

Journal Article · Fri Aug 01 00:00:00 EDT 1986 · Sov. J. Bioorg. Chem. (Engl. Transl.); (United States)

OSTI ID:5242865

Papchikhin, A V; Purygin, P P; Azhaev, A V; Kraevskii, A A; Kutateladze, T V; Chidzhavadze, Z G; Bibilashvili, R Sh

5'-Triphosphates of 3'-azido- and 3'-amino-3'-deoxyarabinonucleosides containing adenine, guanine, cytosine, and thymine residues have been synthesized. Their substrate properties in cell-free systems with E. coli DNA polymerase I, calf thymus DNA polymerase ..cap alpha.., phage T4 DNA polymerase, reverse transcriptase coded by the avian myeloblastosis virus, and also calf thymus terminal deoxynucleotidyl transferase have been studied. It has been shown that aNTP(3'NH/sub 2/) and aNTP(3'-N/sub 3/), act as DNA-chain-terminating substrates for DNA polymerase ..cap alpha.., phage T4 DNA polymerase, reverse transcriptase, and terminal transferase. On the other hand, DNA polymerase I makes practically no use of these compounds as substrates.

• Research Organization: Kuibyshev State Univ., Moscow (USSR)
• OSTI ID: 5242865
• Journal Information: Sov. J. Bioorg. Chem. (Engl. Transl.); (United States), Journal Name: Sov. J. Bioorg. Chem. (Engl. Transl.); (United States) Vol. 11:10; ISSN SJBCD
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
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Molecular & Chemical Physics-- Atomic & Molecular Properties & Theory
74 ATOMIC AND MOLECULAR PHYSICS
ADENINES
AMINES
ANTIMETABOLITES
AROMATICS
AZAARENES
AZINES
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CHEMICAL REACTION YIELD
CHROMATOGRAPHY
COUPLING
COUPLING CONSTANTS
CYTOSINE
DAYS LIVING RADIOISOTOPES
DEUTERIUM COMPOUNDS
DMSO
DNA POLYMERASES
DRUGS
ENZYME ACTIVITY
ENZYMES
ESCHERICHIA COLI
GUANINE
HETEROCYCLIC COMPOUNDS
HYDROGEN 1
HYDROGEN COMPOUNDS
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
HYPERFINE STRUCTURE
INTERMEDIATE COUPLING
ISOTOPES
J-J COUPLING
LIGHT NUCLEI
MATERIALS TESTING
MICROORGANISMS
MOLECULAR STRUCTURE
MULTIPLETS
NMR SPECTRA
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
NUCLEOTIDYLTRANSFERASES
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
POLYMERASES
PURINES
PYRIMIDINES
RADIOENZYMATIC ASSAY
RADIOISOTOPES
RIBOSIDES
SEPARATION PROCESSES
SPECTRA
STABLE ISOTOPES
SUBSTRATES
SULFOXIDES
SYNTHESIS
TESTING
THYMINE
TRANSCRIPTION
TRANSFERASES
URACILS
YIELDS