Inhibition of protein synthesis in maize and wheat by trichothecene mycotoxins and hybridoma-based enzyme immunoassay for deoxynivalenol

Thesis/Dissertation · Thu Jan 01 00:00:00 EST 1987

OSTI ID:5242246

Casale, W L

The 12,13-epoxytrichothecene mycotoxins deoxynivalenol (DON, vomitoxin) and T-2 toxin inhibited protein synthesis in vivo and in cell-free systems from wheat and maize, host plants of trichothecene-producing Fusarium spp.Protein synthesis in tissue (leaf discs and kernel sections) was measured by incorporation of /sup 3/H-leucine into acetone:ethanol insoluble material, and in cell-free translation systems from wheat embryos and maize seedling plumules by incorporation of /sup 3/H-leucine into trichloroacetic acid-insoluble material. The toxin concentration inhibiting 50% of /sup 3/H-leucine incorporation (ID/sub 50/) by several maize varieties were 0.9 ..mu..M (T-2 toxin) and 9-22 ..mu..M (DON). ID/sub 50/ values for wheat were 0.25 ..mu..M (T-2 toxin) and 4.5 ..mu..M (DON).

• Research Organization: Michigan Univ., Ann Arbor (USA)
• OSTI ID: 5242246
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PROTEINS
BIOSYNTHESIS
TOXINS
BIOLOGICAL EFFECTS
ENZYME IMMUNOASSAY
HYBRIDOMAS
INHIBITION
LEUCINE
MAIZE
PLANT TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
WHEAT
AMINO ACIDS
ANIMAL CELLS
ANTIGENS
BODY
CARBOXYLIC ACIDS
CEREALS
GRASS
IMMUNOASSAY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MATERIALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
SYNTHESIS
TISSUES
TOXIC MATERIALS
550201* - Biochemistry- Tracer Techniques