Inhibition of protein synthesis in maize and wheat by trichothecene mycotoxins and hybridoma-based enzyme immunoassay for deoxynivalenol

Thesis/Dissertation · Wed Dec 31 23:00:00 EST 1986

OSTI ID:5242246

Casale, W L

The 12,13-epoxytrichothecene mycotoxins deoxynivalenol (DON, vomitoxin) and T-2 toxin inhibited protein synthesis in vivo and in cell-free systems from wheat and maize, host plants of trichothecene-producing Fusarium spp.Protein synthesis in tissue (leaf discs and kernel sections) was measured by incorporation of /sup 3/H-leucine into acetone:ethanol insoluble material, and in cell-free translation systems from wheat embryos and maize seedling plumules by incorporation of /sup 3/H-leucine into trichloroacetic acid-insoluble material. The toxin concentration inhibiting 50% of /sup 3/H-leucine incorporation (ID/sub 50/) by several maize varieties were 0.9 ..mu..M (T-2 toxin) and 9-22 ..mu..M (DON). ID/sub 50/ values for wheat were 0.25 ..mu..M (T-2 toxin) and 4.5 ..mu..M (DON).

• Research Organization: Michigan Univ., Ann Arbor (USA)
• OSTI ID: 5242246
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANTIGENS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CARBOXYLIC ACIDS
CEREALS
ENZYME IMMUNOASSAY
GRASS
HYBRIDOMAS
IMMUNOASSAY
INHIBITION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUCINE
MAIZE
MATERIALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANT TISSUES
PLANTS
PROTEINS
SYNTHESIS
TISSUES
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
WHEAT