Differential sensitivity of mouse oocytes to colchicine-induced aneuploidy

Journal Article · Thu Jan 01 00:00:00 EST 1987 · Environ. Mutagen.; (United States)

DOI:https://doi.org/10.1002/em.2850100208· OSTI ID:5238013

Mailhes, J B; Yuan, Z P

Unpublished results from our laboratory showed that colchicine increased the incidence of hyperploid mouse metaphase II (MII) oocytes when injected at the same time as human chorionic gonadotrophin (HCG). The objective of the present study was to determine whether the time of administering colchicine influenced the incidence of aneuploidy in MII oocytes. CD-1 mice were given pregnant mare's serum (PMS) and, 48 hr later, HCG. An intraperitoneal injection of 0.2 mg/kg colchicine was given at +4, +2, 0, -2, or -4 hr relative to HCG. Oocytes were collected 17 hr post-HCG and processed, and chromosomes were subsequently C-banded. The percentage of hyperploid oocytes was 0.77, 2.56, 5.71, 7.79, 3.54, and 2.70 for control, +4, +2, 0, -2, or -4 hr pre/post-HCG, respectively. Chi-square analyses of these data demonstrated that colchicine significantly increases the proportion of aneuploid oocytes, and that the relative sensitivity of colchicine-induced aneuploidy depends upon the time that this drug is administered relative to HCG.

• Research Organization: Louisiana State Univ., Shreveport
• OSTI ID: 5238013
• Journal Information: Environ. Mutagen.; (United States), Vol. 10:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
COLCHICINE
BIOLOGICAL EFFECTS
OOCYTES
SENSITIVITY
ANEUPLOIDY
MEIOSIS
MICE
MUTAGENESIS
PREGNANCY
ALKALOIDS
ANIMALS
ANTIMITOTIC DRUGS
ANTIPYRETICS
CELL DIVISION
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DRUGS
GERM CELLS
MAMMALS
ORGANIC COMPOUNDS
PLOIDY
RODENTS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology