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S/sub 1/ nuclease from Aspergillus oryzae for the detection of DNA damage and repair in the gamma-irradiated intracerebral rat gliosarcoma 9L

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3574560· OSTI ID:5237254
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DNA damage and repair in a rat brain tumor following irradiation in vivo were measured by analysis of the rate of strand separation of the tumor DNA in alkali. Tumors were removed after irradiation and mechanically dissociated to a cellular suspension. Tumor cells were injected into alkali (pH 12) for 20 min at 22/sup 0/C. The fraction of tumor DNA remaining double-stranded after this exposure to alkali was determined by its resistance to S/sub 1/ nuclease from Aspergillus oryzae. Double-stranded DNA remains (after enzyme exposure) acid-precipitable for fluorescent assay. The double-stranded fraction after exposure to alkali decreases with increasing radiation dose following first-order kinetics. DNA from tumors excised at intervals after irradiation showed a greater double-stranded fraction in alkali than that from tumors excised immediately, indicating repair of single-strand breaks. Repair of damage produced by 600 rad proceeded with a half-time of approximately 15 min.
Research Organization:
Baltimore Cancer Research Center
OSTI ID:
5237254
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 72:1; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ASPERGILLUS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOMASS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
DISEASES
DNA
ELECTROMAGNETIC RADIATION
ENERGY SOURCES
ENZYMES
FUNGI
GAMMA RADIATION
IONIZING RADIATIONS
MAMMALS
NEOPLASMS
NERVOUS SYSTEM
NUCLEASES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHOSPHOTRANSFERASES
PLANTS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RATS
RECOVERY
RENEWABLE ENERGY SOURCES
REPAIR
RODENTS
STRAND BREAKS
TRANSFERASES
VERTEBRATES