Laser microbeam irradiation and renucleation of mouse eggs. Final progress report, July 1, 1979-December 31, 1983
Technical Report
·
OSTI ID:5233793
We have combined laser microbeam irradiation of mouse egg nuclei with fusion to donor cell nuclei in order to develop a new procedure for transferring nuclei into mammalian eggs. We have been using virus-treated cells injected into the perivitelline for fusion with egg cells. Binucleate cells inside the zona pellucida were often produced indicating nuclear transfer between cells had occurred. To prevent the formation of such abortive polyploidy, host nuclei were inactivated with a laser microbeam. The subsequent cleavage of the microirradiated eggs has been studied.
- Research Organization:
- California Univ., San Francisco (USA). Dept. of Anatomy
- DOE Contract Number:
- AT03-76EV70289
- OSTI ID:
- 5233793
- Report Number(s):
- DOE/EV/70289-T2; ON: DE84006666
- Country of Publication:
- United States
- Language:
- English
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