In vitro stimulation of stage-specific deoxyribonucleic acid synthesis in rat seminiferous tubule segments by interleukin-1. alpha
- Pediatric Endocrinology Unit, Karolinska Hospital, Stockholm (Sweden)
Levels of rat testicular interleukin-1-like factor (tIL-1) have been shown to correlate with DNA synthetic activity during the cycle of the rat seminiferous epithelium, suggesting its role as a spermatogonial or meiotic growth factor. To explore this further, a new in vitro model system was developed. Rat seminiferous tubule segments from stages I, V, VIIa, and VIII-IX of the cycle were isolated by transillumination-assisted microdissection, cultured in chemically defined serum-free medium supplemented with human recombinant IL-1 {alpha}, and labeled with (3H)thymidine. During incubation, spontaneous progression of spermatogenesis was noted. Inactive stage VIIa tubule segments differentiated to stage VIII and initiated DNA synthesis, and concomitantly started to secrete IL-1-like factor. DNA synthesis of stages VIII-IX ceased through differentiation of spermatocytes to leptotene-zygotene (stages XII-XIII of the cycle). IL-1 {alpha} stimulated DNA synthesis significantly in spermatogonia of stage I. Meiotic DNA synthesis at stage VIIa was stimulated (48 h/34 C) and maintained at stages VIII-IX (48 h/34 C). IL-1 {alpha} seems to act as a regulator of spermatogenic DNA synthesis in both mitotic and meiotic phases. It has mainly stimulating and maintaining effects, but it may also be inhibitory under certain conditions.
- OSTI ID:
- 5229688
- Journal Information:
- Endocrinology; (United States), Vol. 129:3; ISSN 0013-7227
- Country of Publication:
- United States
- Language:
- English
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LYMPHOKINES
BIOCHEMICAL REACTION KINETICS
TESTES
DNA REPLICATION
AUTORADIOGRAPHY
CELL CYCLE
EPITHELIUM
IN VITRO
RATS
SPERMATOGENESIS
THYMIDINE
TRITIUM COMPOUNDS
ANIMAL TISSUES
ANIMALS
AZINES
BODY
GAMETOGENESIS
GONADS
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
KINETICS
MALE GENITALS
MAMMALS
MITOGENS
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PYRIMIDINES
REACTION KINETICS
RIBOSIDES
RODENTS
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques