In vitro stimulation of stage-specific deoxyribonucleic acid synthesis in rat seminiferous tubule segments by interleukin-1. alpha

Journal Article · Sun Sep 01 04:00:00 EDT 1991 · Endocrinology; (United States)

DOI:https://doi.org/10.1210/endo-129-3-1614· OSTI ID:5229688

Parvinen, M; Soeder, O M; Mali, P; Froeysa, B R; Ritzen, E M ^[1]

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1. Pediatric Endocrinology Unit, Karolinska Hospital, Stockholm (Sweden)

Levels of rat testicular interleukin-1-like factor (tIL-1) have been shown to correlate with DNA synthetic activity during the cycle of the rat seminiferous epithelium, suggesting its role as a spermatogonial or meiotic growth factor. To explore this further, a new in vitro model system was developed. Rat seminiferous tubule segments from stages I, V, VIIa, and VIII-IX of the cycle were isolated by transillumination-assisted microdissection, cultured in chemically defined serum-free medium supplemented with human recombinant IL-1 {alpha}, and labeled with (3H)thymidine. During incubation, spontaneous progression of spermatogenesis was noted. Inactive stage VIIa tubule segments differentiated to stage VIII and initiated DNA synthesis, and concomitantly started to secrete IL-1-like factor. DNA synthesis of stages VIII-IX ceased through differentiation of spermatocytes to leptotene-zygotene (stages XII-XIII of the cycle). IL-1 {alpha} stimulated DNA synthesis significantly in spermatogonia of stage I. Meiotic DNA synthesis at stage VIIa was stimulated (48 h/34 C) and maintained at stages VIII-IX (48 h/34 C). IL-1 {alpha} seems to act as a regulator of spermatogenic DNA synthesis in both mitotic and meiotic phases. It has mainly stimulating and maintaining effects, but it may also be inhibitory under certain conditions.

• OSTI ID: 5229688
• Journal Information: Endocrinology; (United States), Journal Name: Endocrinology; (United States) Vol. 129:3; ISSN ENDOA; ISSN 0013-7227
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
ANIMALS
AUTORADIOGRAPHY
AZINES
BIOCHEMICAL REACTION KINETICS
BODY
CELL CYCLE
DNA REPLICATION
EPITHELIUM
GAMETOGENESIS
GONADS
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
IN VITRO
KINETICS
LYMPHOKINES
MALE GENITALS
MAMMALS
MITOGENS
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PYRIMIDINES
RATS
REACTION KINETICS
RIBOSIDES
RODENTS
SPERMATOGENESIS
TESTES
THYMIDINE
TISSUES
TRITIUM COMPOUNDS
VERTEBRATES