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Trans-dominant negative mutants of Fos and Jun

Journal Article · · Proceedings of the National Academy of Sciences of the United States of America; (United States)
; ; ;  [1]
  1. Salk Institute, San Diego, CA (USA)
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Jun and Fos nuclear oncoproteins form a complex that regulates transcription from promoters containing activator protein AP-1 binding sites. The leucine-zipper and basic-region domains of both Fos and Jun are necessary for formation of the heterodimer that binds to DNA. Reciprocal mutations in the basic region of Fos or Jun can influence the binding of the heterodimer to DNA, implying a symmetrical binding site. DNA-binding mutants of Jun exhibit increased affinity for Fos and are capable of suppressing wild-type Fos-Jun DNA-binding activity. In contrast, mutations in the basic domain of Fos, which prevent binding to DNA in association with Jun, do not significantly diminish the ability of the wild-type heterodimer to bind to DNA. These dominant negative mutants are functional in vivo and can be exploited to study the role of Fos and Jun in normal and transformed cells.
OSTI ID:
5229509
Journal Information:
Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 87:10; ISSN PNASA; ISSN 0027-8424
Country of Publication:
United States
Language:
English

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550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CARCINOMAS
DAYS LIVING RADIOISOTOPES
DISEASES
DNA HYBRIDIZATION
GENE MUTATIONS
GENE REGULATION
GENE REPRESSORS
GENES
HYBRIDIZATION
ISOTOPE APPLICATIONS
ISOTOPES
LEUCINE
LIGHT NUCLEI
MAMMALS
MAN
MICE
MOLECULAR STRUCTURE
MUTATIONS
NEOPLASMS
NUCLEI
NUCLEOPROTEINS
ODD-ODD NUCLEI
ONCOGENES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
PROTEIN STRUCTURE
PROTEINS
RADIOISOTOPES
RODENTS
TRACER TECHNIQUES
TRANSCRIPTION FACTORS
TUMOR CELLS
VERTEBRATES