A two allele DNA polymorphism of the human phenylethanolamine N-methyltransferase (hPNMT) gene identified by HGIA I

Journal Article · Wed Jan 25 04:00:00 EST 1989 · Nucleic Acids Research; (UK)

DOI:https://doi.org/10.1093/nar/17.2.828· OSTI ID:5229436

Hoehe, M R; Berrettini, W H ^[1]; Baetge, E E ^[2]

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1. NIMH, Bethesda, MD (USA)
2. Univ. of Washington, Seattle (USA)

An 8 kb DNA fragment (Eco RI) of the human phenylethanolamine N-methyltransferase gene (hPNMT), selected from a 14 kb Eco RI fragment isolated from a lambda Charon 3A human lymphocyte genomic library and subcloned into pUC18, was used as a probe. This human genomic fragment contained the first 1,923 bp of 5{prime} flanking DNA, the hPNMT structural gene spanning 2,070 bp in total (composed of three exons (225, 208, 524 bp) and two introns (1,000 and 113 bp)), and 3.8 kb of 3{prime} flanking DNA (1). Hybridization of human genomic DNA digested with HgiA I identifies a two allele polymorphism with bands at 3.0 kb (A) and 2.5 kb (B). The hPNMT gene has been assigned to chromosome 17. Co-dominant segregation in two families with two generations was observed. The number of meioses scorred was 18.

• OSTI ID: 5229436
• Journal Information: Nucleic Acids Research; (UK), Journal Name: Nucleic Acids Research; (UK) Vol. 17:2; ISSN 0305-1048; ISSN NARHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMOSOMES
CLONING
CONNECTIVE TISSUE CELLS
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
ENZYMES
GENETIC MAPPING
HETEROCHROMOSOMES
HUMAN POPULATIONS
HYBRIDIZATION
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MAPPING
MATERIALS
METHYL TRANSFERASES
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
POPULATIONS
PRIMATES
RECOMBINANT DNA
RFLPS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
VERTEBRATES