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Turnover of growth hormone receptors in rat adipocytes

Journal Article · · Endocrinology; (United States)
;

Adipocytes isolated from the epididymal fat pads of normal rats specifically bound (/sup 125/I)human GH (( /sup 125/I)hGH). Preincubation of cells with 20 micrograms/ml cycloheximide, an inhibitor of protein synthesis, produced a progressive loss of ability to bind (/sup 125/I)hGH specifically. Loss of binding sites with time followed first order kinetics and had a half-time of about 45 min regardless of whether GH was present or absent during treatment with cycloheximide. Nonspecific binding of labeled hormone was unchanged by cycloheximide. Similar results were obtained when adipocytes were incubated with 200 micrograms/ml puromycin, another inhibitor of translation, but incubation with 5 micrograms/ml actinomycin D, an inhibitor of transcription, for 2.5 h had no effect on the binding of (/sup 125/I)hGH by adipocytes. The findings are not attributable to cell death, since oxidation of (U-/sup 14/C) glucose to /sup 14/CO/sub 2/ and binding of (/sup 125/I)insulin were unaffected in replicate cell populations exposed to the same treatments. Diminished binding could not be attributed to an effect of cycloheximide to hasten the degradation of receptor-bound hGH. Treatment of adipocytes with 0.1 mg/ml trypsin for 10 min virtually abolished their ability to bind (/sup 125/I)hGH specifically, but binding capability gradually returned after removal of trypsin and was nearly restored to pretrypsin levels by 2 h. Addition of cycloheximide to the incubation medium after removal of trypsin completely prevented recovery of binding capability.

Research Organization:
Univ. of Massachusetts Medical School, Worcester
OSTI ID:
5229402
Journal Information:
Endocrinology; (United States), Journal Name: Endocrinology; (United States) Vol. 5; ISSN ENDOA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADIPOSE TISSUE
ANIMAL TISSUES
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CHALCOGENIDES
CHEMICAL BONDS
CONNECTIVE TISSUE
CYCLOHEXIMIDE
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
FUNGICIDES
HORMONES
HYDROLASES
IN VITRO
INSULIN
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
OXIDES
OXYGEN COMPOUNDS
PEPTIDE HORMONES
PEPTIDE HYDROLASES
PESTICIDES
PITUITARY HORMONES
RADIOISOTOPES
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SERINE PROTEINASES
STH
TISSUES
TRACER TECHNIQUES
TRYPSIN
VERTEBRATES