Liquid chromatographic determination of basic nitrogen-containing polynuclear aromatic hydrocarbons in smoked foods

Conference · · J. Assoc. Off. Anal. Chem.; (United States)

OSTI ID:5229071

Several smoked foods were analyzed for basic nitrogen-containing polynuclear aromatic hydrocarbon (NPAH) content by a relatively rapid liquid chromatographic (LC) technique. Nanogram quantities of NPAH standards were detected by UV and fluorescence detectors connected in series. The NPAHs were extracted from basic aqueous ethanolic solution into cyclohexane, extracted from cyclohexane into 6N HCl, and extracted back into cyclohexane after neutralization of the acid. The NPAHs were then purified by filtering the extract through deactivated basic alumina. The eluate from this step was concentrated to dryness, and the residue was dissolved in 95% ethanol and analyzed by LC, using a Vydac C-18 column and acetonitrile-water (9 + 1) as the mobile phase. Recoveries of 3 NPAHs, 5,7-dimethylbenz(a)acridine, dibenz(a,j)acridine, and dibenz(a,h)acridine each added to salmon and sausage at the 5 ppb level, ranged from 62 to 101% by fluorescence measurement and from 64 to 106% by UV measurement.

• Research Organization: Food and Drug Administration, Washington, DC
• OSTI ID: 5229071
• Conference Information: Journal Name: J. Assoc. Off. Anal. Chem.; (United States) Journal Volume: 69:2
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
ACRIDINES
AROMATICS
AZAARENES
AZINES
CHEMICAL ANALYSIS
CHROMATOGRAPHY
DATA
EXPERIMENTAL DATA
EXTRACTION
FLUORESCENCE
FOOD
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
INFORMATION
LIQUID COLUMN CHROMATOGRAPHY
LUMINESCENCE
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
POLYCYCLIC AROMATIC HYDROCARBONS
PYRIDINES
QUANTITATIVE CHEMICAL ANALYSIS
QUANTITY RATIO
SEPARATION PROCESSES
SOLVENT EXTRACTION
SPECTRA
ULTRAVIOLET SPECTRA