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Title: Fatty acid uptake in normal human myocardium

Abstract

Fatty acid binding protein has been found in rat aortic endothelial cell membrane. It has been identified to be a 40-kDa protein that corresponds to a 40-kDa fatty acid binding protein with high affinity for a variety of long chain fatty acids isolated from rat heart myocytes. It is proposed that this endothelial membrane fatty acid binding protein might mediate the myocardial uptake of fatty acids. For evaluation of this hypothesis in vivo, influx kinetics of tracer-labeled fatty acids was examined in 15 normal subjects by scintigraphic techniques. Variation of the plasma fatty acid concentration and plasma perfusion rate has been achieved by modulation of nutrition state and exercise conditions. The clinical results suggest that the myocardial fatty acid influx rate is saturable by increasing fatty acid plasma concentration as well as by increasing plasma flow. For analysis of these data, functional relations describing fatty acid transport from plasma into myocardial tissue in the presence and absence of an unstirred layer were developed. The fitting of these relations to experimental data indicate that the free fatty acid influx into myocardial tissue reveals the criteria of a reaction on a capillary surface in the vicinity of flowing plasma but not ofmore » a reaction in extravascular space or in an unstirred layer and that the fatty acid influx into normal myocardium is a saturable process that is characterized by the quantity corresponding to the Michaelis-Menten constant, Km, and the maximal velocity, Vmax, 0.24 {plus minus} 0.024 mumol/g and 0.37 {plus minus} 0.013 mumol/g(g.min), respectively. These data are compatible with a nondiffusional uptake process mediated by the initial interaction of fatty acids with the 40-kDa membrane fatty acid binding protein of cardiac endothelial cells.« less

Authors:
; ; ; ; ; ; ; ;  [1]
  1. (University of Bochum (West Germany))
Publication Date:
OSTI Identifier:
5228784
Resource Type:
Journal Article
Resource Relation:
Journal Name: Circulation Research (An Official Journal of the American Heart Association); (United States); Journal Volume: 69:3
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; CARBOXYLIC ACIDS; METABOLISM; MYOCARDIUM; PROTEINS; BIOSYNTHESIS; AORTA; CELL MEMBRANES; DYNAMIC FUNCTION STUDIES; ENDOTHELIUM; EXERCISE; MAN; SCINTISCANNING; THALLIUM ISOTOPES; ANIMAL TISSUES; ANIMALS; ARTERIES; BLOOD VESSELS; BODY; CARDIOVASCULAR SYSTEM; CELL CONSTITUENTS; COUNTING TECHNIQUES; DIAGNOSTIC TECHNIQUES; HEART; ISOTOPES; MAMMALS; MEMBRANES; MUSCLES; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PRIMATES; RADIOISOTOPE SCANNING; SYNTHESIS; TISSUES; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics

Citation Formats

Vyska, K., Meyer, W., Stremmel, W., Notohamiprodjo, G., Minami, K., Machulla, H.J., Gleichmann, U., Meyer, H., and Koerfer, R. Fatty acid uptake in normal human myocardium. United States: N. p., 1991. Web. doi:10.1161/01.RES.69.3.857.
Vyska, K., Meyer, W., Stremmel, W., Notohamiprodjo, G., Minami, K., Machulla, H.J., Gleichmann, U., Meyer, H., & Koerfer, R. Fatty acid uptake in normal human myocardium. United States. doi:10.1161/01.RES.69.3.857.
Vyska, K., Meyer, W., Stremmel, W., Notohamiprodjo, G., Minami, K., Machulla, H.J., Gleichmann, U., Meyer, H., and Koerfer, R. Sun . "Fatty acid uptake in normal human myocardium". United States. doi:10.1161/01.RES.69.3.857.
@article{osti_5228784,
title = {Fatty acid uptake in normal human myocardium},
author = {Vyska, K. and Meyer, W. and Stremmel, W. and Notohamiprodjo, G. and Minami, K. and Machulla, H.J. and Gleichmann, U. and Meyer, H. and Koerfer, R.},
abstractNote = {Fatty acid binding protein has been found in rat aortic endothelial cell membrane. It has been identified to be a 40-kDa protein that corresponds to a 40-kDa fatty acid binding protein with high affinity for a variety of long chain fatty acids isolated from rat heart myocytes. It is proposed that this endothelial membrane fatty acid binding protein might mediate the myocardial uptake of fatty acids. For evaluation of this hypothesis in vivo, influx kinetics of tracer-labeled fatty acids was examined in 15 normal subjects by scintigraphic techniques. Variation of the plasma fatty acid concentration and plasma perfusion rate has been achieved by modulation of nutrition state and exercise conditions. The clinical results suggest that the myocardial fatty acid influx rate is saturable by increasing fatty acid plasma concentration as well as by increasing plasma flow. For analysis of these data, functional relations describing fatty acid transport from plasma into myocardial tissue in the presence and absence of an unstirred layer were developed. The fitting of these relations to experimental data indicate that the free fatty acid influx into myocardial tissue reveals the criteria of a reaction on a capillary surface in the vicinity of flowing plasma but not of a reaction in extravascular space or in an unstirred layer and that the fatty acid influx into normal myocardium is a saturable process that is characterized by the quantity corresponding to the Michaelis-Menten constant, Km, and the maximal velocity, Vmax, 0.24 {plus minus} 0.024 mumol/g and 0.37 {plus minus} 0.013 mumol/g(g.min), respectively. These data are compatible with a nondiffusional uptake process mediated by the initial interaction of fatty acids with the 40-kDa membrane fatty acid binding protein of cardiac endothelial cells.},
doi = {10.1161/01.RES.69.3.857},
journal = {Circulation Research (An Official Journal of the American Heart Association); (United States)},
number = ,
volume = 69:3,
place = {United States},
year = {Sun Sep 01 00:00:00 EDT 1991},
month = {Sun Sep 01 00:00:00 EDT 1991}
}