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Multiple isoelectric forms of poliovirus RNA-dependent RNA polymerase: Evidence for phosphorylation

Journal Article · · Journal of Virology; (USA)
OSTI ID:5228659
;  [1]
  1. Univ. of California, Los Angeles (USA)
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Poliovirus-specific RNA-dependent RNA polymerase (3Dpol) was purified to apparent homogeneity. A single polypeptide of an apparent molecular weight of 63,000 catalyzes the synthesis of dimeric and monomeric RNA products in response to the poliovirion RNA template. Analysis of purified 3Dpol by two-dimensional electrophoresis showed multiple forms of 3Dpol, suggesting posttranslational modification of the protein in virus-infected cells. The two major forms of 3Dpol appear to have approximate pI values of 7.1 and 7.4. Incubation of purified 3Dpol with calf intestinal phosphatase resulted in almost complete disappearance of the pI 7.1 form and a concomitant increase in the intensity of the pI 7.4 form of 3Dpol. Addition of 32P-labeled Pi during infection of HeLa cells with poliovirus resulted in specific labeling of 3Dpol and 3CD, a viral protein which contains the entire 3Dpol sequence. Both 3Dpol and 3CD appear to be phosphorylated at serine residues. Ribosomal salt washes prepared from both mock- and poliovirus-infected cells contain phosphatases capable of dephosphorylating quantitatively the phosphorylated form (pI 7.1) of 3Dpol.
OSTI ID:
5228659
Journal Information:
Journal of Virology; (USA), Journal Name: Journal of Virology; (USA) Vol. 63:11; ISSN 0022-538X; ISSN JOVIA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ELECTROPHORESIS
ENZYMES
ESTERASES
HELA CELLS
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
MICROORGANISMS
MOLECULAR STRUCTURE
MOLECULAR WEIGHT
NUCLEI
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
PARASITES
PHOSPHATASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
POLIO VIRUS
POLYMERASES
PRIMATES
RADIOISOTOPES
RNA POLYMERASES
TRACER TECHNIQUES
TRANSCRIPTION
TRANSFERASES
TWO-DIMENSIONAL ELECTROPHORESIS
VERTEBRATES
VIRUSES