Plasminogen activator activity in lung and alveolar macrophages of rats exposed to graded single doses of. gamma. rays to the right hemithorax
Male rats were sacrificed 2 or 6 months after a single dose of 0-30 Gy of /sup 60/Co ..gamma.. rays to the right hemithorax. At autopsy, macrophages were lavaged from the right lung, counted, and frozen. The right (irradiated) and the left (shielded) lungs were frozen, then assayed for plasminogen activator (PLA) activity by the fibrin plate lysis method. Freeze-thawed macrophages were assayed for both PLA activity (/sup 125/I-fibrin clot lysis method) and fibrinolytic inhibitor activity (inhibition of urokinase-induced fibrin lysis). There was a linear, dose-dependent decrease in right lung PLA activity over the dose range of 10-30 Gy at 2 and 6 months postirradiation, reductions of 3.1 and 2.6% per Gy, respectively. PLA activity at all radiation doses was 10-15% higher at 6 months than at 2 months indicative of a partial recovery of this endothelial function in the irradiated lung. PLA activity per 10/sup 6/ macrophages decreased with increasing radiation dose at both autopsy times, closely paralleling lung PLA activity. This radiation-induced decrease in macrophage PLA activity was not due to increased fibrinolytic inhibitor activity in the irradiated macrophages. These data quantitate the dose response and time course of radiation-induced fibrinolytic defects in rat lung and suggest that information obtained from a minimally invasive procedure such as bronchoalveolar lavage may serve as an index of the degree of pulmonary fibrinolytic dysfunction after irradiation.
- Research Organization:
- Northwestern Univ., Chicago, IL
- OSTI ID:
- 5228075
- Journal Information:
- Radiat. Res.; (United States), Vol. 103:3
- Country of Publication:
- United States
- Language:
- English
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FIBRINOLYSIS
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LUNGS
BIOLOGICAL RADIATION EFFECTS
MACROPHAGES
AUTOPSY
COBALT 60
DOSE-RESPONSE RELATIONSHIPS
GAMMA RADIATION
IODINE 125
PLASMINOGEN
RATS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
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BODY
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COBALT ISOTOPES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DIAGNOSTIC TECHNIQUES
DRUGS
ELECTROMAGNETIC RADIATION
ELECTRON CAPTURE RADIOISOTOPES
FIBRINOLYTIC AGENTS
HEMATOLOGIC AGENTS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE ISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANS
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RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
VERTEBRATES
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560121* - Radiation Effects on Cells- External Source- (-1987)