Estimation of plasma tacrine concentrations using an in vitro cholinesterase inhibition assay

Journal Article · · Alzheimer Disease and Associated; (USA)

DOI:https://doi.org/10.1097/00002093-198903030-00003· OSTI ID:5225411

Moriearty, P L; Kenny, W; Kumar, V ^[1]

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1. Southern Illinois Univ. School of Medicine, Springfield (USA)

THA (9-amino, 1,2,3,4-tetrahydroacridine; tacrine) is currently under study as a cholinesterase (ChE) inhibitor in Alzheimer disease. In this study, a sensitive radiometric assay for THA inhibition of human plasma ChE, suitable for detection of effects of orally administered drug, is described. The assay is sensitive in a range of 4-50 ng/ml plasma. Reversibility of the inhibition permits distinguishing of drug effects on ChE from changes in amount of enzyme synthesized during treatment.

• OSTI ID: 5225411
• Journal Information: Alzheimer Disease and Associated; (USA), Vol. 3:3; ISSN 0893-0341
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ENZYME INHIBITORS
RADIOASSAY
NERVOUS SYSTEM DISEASES
CHEMOTHERAPY
CHOLINESTERASE
MAN
ORAL ADMINISTRATION
TRACER TECHNIQUES
ANIMALS
CARBOXYLESTERASES
DISEASES
ENZYMES
ESTERASES
HYDROLASES
ISOTOPE APPLICATIONS
MAMMALS
PRIMATES
THERAPY
VERTEBRATES
550901* - Pathology- Tracer Techniques