Enzymatic coupling of 2,4-dichlorophenol to stream fulvic acid in the presence of oxidoreductases
- Pennsylvania State Univ., Univ. Park (USA)
The coupling {sup 14}C-ring-labelled 2,4-dichlorophenol (2,4-DCP) to stream fulvic acid was investigated in the presence of several oxidoreductases including tyrosinase, peroxidase, and laccases of Rhizoctonia praticola and Trametes vesicolor. During 12-h incubation of the oxidoreductases with {sup 14}C-2, 4-DCP and stream fulvic acid, a substantial amount of the radioactivity was incorporated into fulvic acid. Chromatographic analysis indicated that although a large portion of the radioactivity remained in solution, no unbound {sup 14}C-2,4-DCP was present in the supernatant. The effects of pH, temperature, concentration of fulvic acid, and concentration of enzyme on the coupling processes were studied. The results of this research provide evidence that the enzymatic coupling of certain xenobiotic pollutants to humic substances is an important natural process which must be considered in studies of the fate, reactivity, and persistence of these organic compounds in soils and stream waters.
- OSTI ID:
- 5222765
- Report Number(s):
- CONF-881108--
- Journal Information:
- Soil Science Society of America Journal; (USA), Journal Name: Soil Science Society of America Journal; (USA) Vol. 52:3; ISSN SSSJD; ISSN 0361-5995
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
540320 -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (1990-)
550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BIODEGRADATION
CARBON 14 COMPOUNDS
CHEMICAL REACTION KINETICS
CHEMICAL REACTIONS
CHLORINATED AROMATIC HYDROCARBONS
CHROMATOGRAPHY
DECOMPOSITION
DETOXIFICATION
ENZYME ACTIVITY
ENZYMES
FULVIC ACIDS
FUNGI
HALOGENATED AROMATIC HYDROCARBONS
ION EXCHANGE
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
ORGANIC ACIDS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXIDOREDUCTASES
PH VALUE
PLANTS
REACTION KINETICS
SEPARATION PROCESSES
STREAMS
SURFACE WATERS
TEMPERATURE EFFECTS
TRACER TECHNIQUES