Effect of the dB-c-AMP and forskolin on /sup 45/Ca influx, net Ca uptake and tension on rabbit aortic smooth muscle

Conference · Sat Mar 01 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5220104

The effect of dibutiryl-adenosine-3',5'-cyclic-monophosphate (dB-c-AMP) and forskolin on aortic tension and /sup 45/Ca influx were measured. dB-c-AMP reduced both the rate of force development and the maximal tension achieved in solutions containing various K/sup +/ concentrations. Stimulated /sup 45/Ca influx was also reduced however to a lesser extent than was the tension. Forskolin showed more marked effects of a similar nature. Thus, both these agents which increase intracellular c-AMP caused a rightward shift in the curve expressing force(ordinate) as a function of Ca influx (abscissa). Consequently, they found that dB-c-AMP stimulated more net Ca to be taken up by the sarcoplasmic reticulum(SR) at the same influx rate. The conclusion that c-AMP produced these effects by stimulating Ca uptake into the superficial SR was supported by the finding that dB-c-AMP increased the amount of Ca taken up into a caffeine releasable fraction.

• Research Organization: Univ. of Miami, FL
• OSTI ID: 5220104
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AMP
BIOLOGICAL EFFECTS
CALCIUM
MEMBRANE TRANSPORT
AORTA
CALCIUM 45
POTASSIUM
SARCOPLASMIC RETICULUM
TRACER TECHNIQUES
ALKALI METALS
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
ARTERIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD VESSELS
BODY
CALCIUM ISOTOPES
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
METALS
NUCLEI
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
RADIOISOTOPES
550201* - Biochemistry- Tracer Techniques