Isolation of differentiated membrane domains from Escherichia coli and Salmonella typhimurium, including a fraction containing sites between the inner and outer membranes and Murein skeleton of the cell envelope

Journal Article · Sun Jan 05 04:00:00 EST 1986 · J. Biol. Chem.; (United States)

OSTI ID:5219959

Cell envelopes of Salmonella typhimurium and Escherichia coli were disrupted in a French pressure cell and fractionated by successive cycles of sedimentation and floatation density gradient centrifugation. This permitted the identification and isolation of several membrane fractions in addition to the major inner membrane and murein-outer membrane fractions. One of these fractions (fraction OM/sub L/) accounted for about 10% of the total cell envelope protein, and is likely to include the murein-membrane adhesion zones that are seen in electron micrographs of plasmolyzed cells. Fraction OM/sub L/ contained inner membrane, murein, and outer membrane in an apparently normal configuration, was capable of synthesizing murein from UDP-(/sup 3/H)N-acetylglucosamine and UDP-N-acetylmuramyl-pentapeptide and covalently linking it to the endogenous murein of the preparation. It showed a labeling pattern in (/sup 3/H)galactose pulse-chase experiments that was consistent with its acting as an intermediate in the movement of newly synthesized lipopolysaccharide from inner membrane to outer membrane.

• Research Organization: Univ. of Connecticut Health Center, Farmington
• OSTI ID: 5219959
• Journal Information: J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 261:1; ISSN JBCHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550701 -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOCHEMICAL REACTION KINETICS
CARBOHYDRATES
CELL CONSTITUENTS
CELL MEMBRANES
DATA
ESCHERICHIA COLI
EXPERIMENTAL DATA
INFORMATION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIPIDS
LIPOPOLYSACCHARIDES
MEMBRANES
MICROORGANISMS
NUMERICAL DATA
ORGANIC COMPOUNDS
POLYSACCHARIDES
PROTEINS
REACTION KINETICS
SACCHARIDES
SALMONELLA
SALMONELLA TYPHIMURIUM
SEPARATION PROCESSES
TRACER TECHNIQUES
TRITIUM COMPOUNDS