Stability of alginate-immobilized algal cells
Investigations were carried out using immobilized Chlorella cells to determine the diameter, compressibility, tolerance to phosphate chelation, and ability to retain algal cells during incubation of various alginate beads. These physical bead-characteristics were affected by a variety of interactive factors, including multivalent cation type (hardening agent) and cell, cation, and alginate concentration, the latter exhibiting a predominant influence. The susceptibility of alginate beads to phosphate chelation involved a complex interaction of cation type, concentration, and pH of phosphate solution. A scale of response ranging from gel swelling to gel shrinking was observed for a range of conditions. However, stable Ca alginate beads were maintained in incubation media with a pH of 5.5 and a phosphate concentration of 5 micro M. A preliminary investigation into cell leakage from the beads illustrated the importance of maintaining a stable gel structure and limiting cell growth to reduce leakage.
- Research Organization:
- School of Natural Science, Hatfield/Herts, England
- OSTI ID:
- 5218521
- Journal Information:
- Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 28:2; ISSN BIBIA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ALGAE
ALGINATES
CATIONS
CHARGED PARTICLES
CHELATING AGENTS
CHLORELLA
COLLOIDS
COMPRESSIBILITY
DISPERSIONS
GELS
GROWTH
IMMOBILIZED CELLS
INCUBATION
IONS
LEAKS
MECHANICAL PROPERTIES
MICROORGANISMS
OXYGEN COMPOUNDS
PH VALUE
PHOSPHATES
PHOSPHORUS COMPOUNDS
PLANT CELLS
PLANTS
STABILITY
UNICELLULAR ALGAE