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Systemic administration of recombinant interleukin 2 stimulates in vivo lymphoid cell proliferation in tissues

Journal Article · · J. Immunol.; (United States)
OSTI ID:5218263
; ; ;

Recent work in the authors laboratory has demonstrated that the repeated injections of high doses of recombinant interleukin 2 (IL 2) can dramatically reduce the number of established pulmonary and hepatic metastases and the growth of intradermal tumors in a variety of murine tumor models. They have thus undertaken studies to define the mechanisms underlying these in vivo effects of IL 2. Using an in vivo DNA-labeling technique in which the authors employed 5-(/sup 125/I)iodo-2'-deoxyuridine (/sup 125/IUdR), they examined the in vivo cell proliferation in the tissues of mice treated with IL 2. A proliferation index (PI) was calculated by dividing the raw counts per minute (cpm) of tissues in IL 2-treated mice by the cpm in corresponding tissues of control animals. At an IL 2 dose of 6000 U given i.p. three times a day, the highest /sup 125/IUdR incorporation was seen in the lungs, liver, spleen, kidneys, and mesenteric lymph nodes (PI = 6.9, 6.9, 5.1, 7.1, 24.6, respectively, at 5 days). The amount of lymphoid proliferation in these organs was a direct function of the dose of IL 2 administered. Other tissues including thymus, intestines, skin, and hind limb showed no significant increase in /sup 125/IUdR uptake even after host treatment with the highest doses of IL 2. Blood and brain demonstrated intermediate incorporation of the radiolabel. Preirradiation of the host largely eliminated the proliferative response to IL 2. Histologic studies of normal and irradiated mice receiving IL 2 corroborated the result of the /sup 125/IUdR findings.

Research Organization:
National Cancer Institute, Bethesda, MD
OSTI ID:
5218263
Journal Information:
J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 2; ISSN JOIMA
Country of Publication:
United States
Language:
English

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Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY AREAS
BODY FLUIDS
BRAIN
CELL PROLIFERATION
CENTRAL NERVOUS SYSTEM
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
DOSE-RESPONSE RELATIONSHIPS
ELECTRON CAPTURE RADIOISOTOPES
GASTROINTESTINAL TRACT
GLANDS
IN VIVO
INTERMEDIATE MASS NUCLEI
INTESTINES
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
LEGS
LEUKOCYTES
LIMBS
LIVER
LUNGS
LYMPH NODES
LYMPHATIC SYSTEM
LYMPHOCYTES
LYMPHOKINES
MAMMALS
MATERIALS
METASTASES
MICE
NEOPLASMS
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RADIOISOTOPES
RESPIRATORY SYSTEM
RODENTS
SKIN
SOMATIC CELLS
SPLEEN
THYMUS
TISSUES
TRACER TECHNIQUES
VERTEBRATES