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Effects of condensation products of biogenic amines on human platelet function

Thesis/Dissertation ·
OSTI ID:5217154
Condensation products (CP) are formed by the reaction of biogenic amines with aldehydes and alpha-keto acids. The purpose of this investigation was to examine the effects of CP on platelet function in vitro. The effect of CP on platelet aggregation was examined. Epinephrine-induced aggregation was inhibited, suggesting CP antagonistic activity on the platelet alpha/sub 2/-adrenergic receptors. Adenosine-diphosphate (ADP), collagen and arachidonic acid induced aggregation was inhibited only at high concentrations. Inhibition of epinephrine and ADP aggregation was reversible, suggesting CP are competitive inhibitors of these agonists. Binding affinities for the platelet alpha/sub 2/-adrenergic receptor were determined using (/sup 3/H)-yohimbine, a specific alpha/sup 2/-receptor antagonist. The order of potency for CP inhibition of (/sup 3/H)-yohimbine binding paralleled that determined for inhibition of epinephrine-induced aggregation. Platelet uptake of serotonin (5-HT) was competitively inhibited by CP, with the exception of salsolinol, which appears to be stimulatory. Release of 5-HT from platelets was induced by CP, with betacarbolines being more potent than tetrahydroisoquinolines. Evidence suggests that CP cause release by displacement of 5-HT from intraplatelet storage sites since this effect can be inhibited by imipramine, thus preventing accumulation of CP by platelets.
Research Organization:
University of South Alabama, Mobile (USA)
OSTI ID:
5217154
Country of Publication:
United States
Language:
English

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Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADP
ADRENAL HORMONES
ADRENALINE
AFFINITY
ALDEHYDES
AMINES
ARACHIDONIC ACID
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CARBOXYLIC ACIDS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CHEMICAL BONDS
COLLAGEN
DRUGS
DYNAMIC FUNCTION STUDIES
HORMONES
IN VITRO
INHIBITION
ISOTOPE APPLICATIONS
KETO ACIDS
LABELLED COMPOUNDS
MATERIALS
MONOCARBOXYLIC ACIDS
NEUROREGULATORS
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
RECEPTORS
SCLEROPROTEINS
STEROID HORMONES
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRITIUM COMPOUNDS