Incorporation of glucose carbons into rat lung lipids after exposure to 0. 6 ppm ozone
Continuous exposure to low concentrations of ozone has previously been associated with proliferation of lung alveolar type II epithelial cells. In this study, /sup 14/C incorporation into tissue lipids was determined in isolated rat lungs by perfusion with (U-/sup 14/C)glucose, at a time of maximal hyperplasia brought about by 3 days continuous exposure to 0.6 ppm ozone. Ozone exposed lungs exhibited increased rates of glycolytic energy production, indicated by an 89% increase in /sup 3/H/sub 2/O generation on perfusion with (5-3H)glucose. Ozone exposure resulted in enhanced /sup 14/C incorporations into glyceride-glycerol and fatty acid moieties of lung lipids of 95 and 180%, respectively, with a greater proportion of label being recovered in shorter chain fatty acids. Although increased labeling was observed in both neutral and phospholipids, the pattern of /sup 14/C recovery suggested a relative increased glucose carbon incorporation into lung free fatty acids, phosphatidic acid, and such membrane associated lipids as phosphatidylinositol and those containing sphingosine. These results are consistent with the needs of a dividing cell population for enhanced energy production and synthesis of new lipids.
- Research Organization:
- Johns Hopkins Univ., Baltimore, MD
- OSTI ID:
- 5216470
- Journal Information:
- Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States); ISSN AJPHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
ANIMALS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CHEMICAL REACTIONS
CHRONIC EXPOSURE
DECOMPOSITION
ESTERS
GLUCOSE
GLYCOLYSIS
HEXOSES
LABELLED COMPOUNDS
LIPIDS
LUNGS
MAMMALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
OZONE
PHOSPHOLIPIDS
RATS
RESPIRATORY SYSTEM
RODENTS
SACCHARIDES
SYNTHESIS
TRITIUM COMPOUNDS
VERTEBRATES