Repair of damaged DNA in-vivo. Comprehensive progress report, August 1980-August 1983
We have extended our characterization of long patch excision repair (LPER) and have demonstrated that LPER is not mutagenic (or error-prone); that the recA function is required for LPER, at least for its regulation; that the substrate for LPER is produced as a linear (not an exponential) function of uv (254 nm) dose; and that LPER can occur in uvr/sup -/ cells treated with N-methyl-N-nitro-N-nitrosoguanidine (MNNG). We have developed 3 methods for measuring the frequency of interstrand crosslinks in DNA and are now applying these methods to the study of the formation and repair of DNA crosslinks in E.Coli. We have developed a monoclonal antibody specific for thymine glycol in DNA, and are using it to study the repair of thymine glycol in E. coli.
- Research Organization:
- Stanford Univ., CA (USA)
- DOE Contract Number:
- AT03-76EV70007
- OSTI ID:
- 5213215
- Report Number(s):
- DOE/EV/70007-T1; ON: DE84006020
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BIOLOGICAL RADIATION EFFECTS
BIOCHEMISTRY
BIOLOGICAL REPAIR
BIOSYNTHESIS
MUTATIONS
NUCLEASES
BIOCHEMICAL REACTION KINETICS
CROSS-LINKING
DNA POLYMERASES
DNA-CLONING
ESCHERICHIA COLI
MONOCLONAL ANTIBODIES
NITROSAMINES
PSORALEN
ULTRAVIOLET RADIATION
AMINES
ANTIBODIES
ANTICOAGULANTS
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
CHEMICAL REACTIONS
CHEMISTRY
CLONING
COUMARINS
DRUGS
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERASES
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
HYDROLASES
KINETICS
MICROORGANISMS
NITROSO COMPOUNDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PHOSPHODIESTERASES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
POLYMERIZATION
RADIATION EFFECTS
RADIATIONS
REACTION KINETICS
RECOVERY
REPAIR
SYNTHESIS
TRANSFERASES
550401* - Genetics- Tracer Techniques
550201 - Biochemistry- Tracer Techniques