Comparison of alveolar and interstitial macrophages in fibroblast stimulation after silica and long or short asbestos

Conference · Fri Mar 15 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5212872

Adamson, I Y.R.; Bowden, D H ^[1]

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1. Univ. of Manitoba, Winnipeg (Canada)

Pulmonary fibrosis in response to particles has been attributed to secretion of fibroblast growth factors (FGF) by alveolar macrophages (AM). However, since fibrosis is interstitial and is associated with particle retention by interstitial macrophages (IM), the authors have now compared the secretory activity of FGF by rat alveolar (AM) and interstitial macrophages (IM) in response to silica and to long or short asbestos fibers. AM were obtained by broncho-alveolar lavage, and IM by collecting macrophages that migrate from explants of a previously lavaged and perfused lung. Isolated Am and IM from fibrotic lungs, 6 weeks after instilling silica, secreted equal amounts of FGF. Six weeks after giving short asbestos fibers in vivo, lavaged AM secreted FGF in vitro, but there was no change in fibroblast growth and no fibrosis in vivo. After giving long fibers, which reach the interstitium, isolated IM secreted FGF and collagen levels were increased in whole lung. When macrophages were isolated from normal rats and exposed to particles in vitro, Am and IM supernatants contained equal amounts of FGF. The results show that these macrophage populations respond equally to particles with respect to FGF secretion. The fibrotic reaction seen in vivo is likely due to the close proximity to fibroblasts to particle-laden macrophages within the interstitium allowing more efficient transfer of growth factors.

• OSTI ID: 5212872
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:5
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ASBESTOS
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BODY
CHALCOGENIDES
COMPARATIVE EVALUATIONS
CONNECTIVE TISSUE CELLS
DISEASES
EVALUATION
FIBROBLASTS
GROWTH FACTORS
LAVAGE
LUNGS
MACROPHAGES
MAMMALS
MITOGENS
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
PHAGOCYTES
PROTEINS
RATS
RESPIRATORY SYSTEM
RESPIRATORY SYSTEM DISEASES
RETENTION
RODENTS
SECRETION
SILICON COMPOUNDS
SILICON OXIDES
SOMATIC CELLS
VERTEBRATES