Glucose metabolite patterns as markers of functional differentiation in freshly isolated and cultured mouse mammary epithelial cells
In the mammary gland of non-ruminant animals, glucose is utilized in a characteristic and unique way during lacation. By measuring the incorporation of glucose carbon from (U-/sup 14/C)glucose into intermediary metabolitees and metabolic products in mammary epithelia cells from virgin, pregnant, and lacating mice, we domonstrate that glucose metabolite patterns can be used to recognize stages of differentiated function. For these cells, the rates of synthesis of glycogen and lactose, the ratio of lactate to alanine, and the ratio of citrate to malate are important parameters in identifying the degree of expression of differentiation. We further show that these patterns can be used as markers to determine the differentiated state of cultured mammary epithelial cells. Cells maintained on plastic substrates lose their distinctive glucose metabolite patterns while those on floating collagen gels do not. Cells isolated from pregnant mice and cultured on collagen gels have a pattern similar to that of their freshly isolated counter-parts. When isolated from lacating mice, the metabolite patterns of cells cultured on collagen gels are different from that of the cells of origin, and resembles that of freshly isolated cells from pregnant mice. Our findings suggest that the floating collagen gels under the culture conditions used in these experiments provide an environment for the functional expression of the pregnant state, while additional factors are needed for the expression of the lactating state.
- Research Organization:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 5211964
- Journal Information:
- Exp. Cell Res.; (United States), Vol. 134
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CELL DIFFERENTIATION
BIOLOGICAL INDICATORS
GLUCOSE
METABOLITES
GLYCOGEN
BIOSYNTHESIS
LACTOSE
ANIMAL CELLS
CARBON 14 COMPOUNDS
CELL CULTURES
CITRATES
COLLAGEN
EPITHELIUM
LACTATION
MAMMARY GLANDS
MICE
PREGNANCY
ALDEHYDES
ANIMAL TISSUES
ANIMALS
BODY
CARBOHYDRATES
CARBOXYLIC ACID SALTS
DISACCHARIDES
GLANDS
HEXOSES
LABELLED COMPOUNDS
MAMMALS
MONOSACCHARIDES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
POLYSACCHARIDES
PROTEINS
RODENTS
SACCHARIDES
SCLEROPROTEINS
SYNTHESIS
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
550301 - Cytology- Tracer Techniques