Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Lymphotoxin cytotoxicity, a combination of cytolytic and cytostatic cellular responses

Journal Article · · Immunopharmacology; (United States)
;
Guinea pig lymphotoxin inhibits the growth of mouse alpha L929 and guinea pig 104C1 tumor cells with lethal doses (LD 50's) of 0.4 and 200 units lymphotoxin/ml, respectively, in a colony inhibition assay. Refeeding with lymphotoxin-free medium is followed by resumption of 104C1 but not alpha L929 cell growth. This suggests that growth inhibition of alpha L929 is primarily due to cytolytic mechanisms, while that of 104C1 cells is due to cytostatic mechanisms. This is confirmed by radionuclide (3H, 51Cr, and 75Se) release assays with LD 50's of 1.0, 1.9, and 2.4 units lymphotoxin/ml, respectively, for alpha L929 cells, whereas as many as 100 units lymphotoxin/ml produce no radionuclide release from 104C1 cells. The L cell variant L929M is 10-fold more resistant to lymphotoxin colony inhibitory activity and 40-300-fold more resistant to cytolytic lymphotoxin activity as measured by the three radionuclide release assays than are alpha L929 cells. L929 and 2071 L cell variants are more resistant as a result of smaller cytolytic and cytostatic responses and some tumor cells, such as one strain of L1210 mouse leukemia cells, exhibit no detectable cytolytic or cytostatic responses to 100 units of guinea pig lymphotoxin. These observations demonstrate that the divergent susceptibilities of different cells to lymphotoxin result in part from constitutive variations in cellular cytolytic and reversible cytostatic responses to lymphotoxin.
Research Organization:
Tumor Biology Section, Laboratory of Biology, Division of Cancer Cause and Prevention, National Cancer Institute, Bethesda, Maryland
OSTI ID:
5211623
Journal Information:
Immunopharmacology; (United States), Journal Name: Immunopharmacology; (United States) Vol. 3:4; ISSN IMMUD
Country of Publication:
United States
Language:
English

Similar Records

Identification of beta-lymphotoxin as the predominant molecular class of in vitro and in vivo Syrian hamster lymphotoxin
Journal Article · Fri Dec 31 23:00:00 EST 1982 · Cell. Immunol.; (United States) · OSTI ID:6253259
Purification and characterization of an inhibitor (soluble tumor necrosis factor receptor) for tumor necrosis factor and lymphotoxin obtained from the serum ultrafiltrates of human cancer patients
Journal Article · Wed Oct 31 23:00:00 EST 1990 · Proceedings of the National Academy of Sciences of the United States of America; (United States) · OSTI ID:5822267
Comparison of various assays to quantitate macrophage activation by biological response modifiers
Journal Article · Sat Dec 31 23:00:00 EST 1983 · J. Immunolopharmacol.; (United States) · OSTI ID:5483582

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL DIVISION
CHROMIUM 51
CHROMIUM ISOTOPES
COLONY FORMATION
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DOSES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
FIBROBLASTS
GUINEA PIGS
IMMUNE REACTIONS
IMMUNOLOGY
INHIBITION
INTERMEDIATE MASS NUCLEI
ISOTOPES
LABELLED COMPOUNDS
LETHAL DOSES
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
NUCLEI
RADIOIMMUNOLOGY
RADIOISOTOPES
RODENTS
SELENIUM 75
SELENIUM ISOTOPES
SOMATIC CELLS
TOXIC MATERIALS
TOXICITY
TOXINS
TRITIUM COMPOUNDS
TUMOR CELLS
VERTEBRATES