Cell survival kinetics in peripheral blood and bone marrow during total body irradiation for marrow transplantation

Journal Article · Tue Nov 01 04:00:00 EST 1983 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

DOI:https://doi.org/10.1016/0360-3016(83)90413-3· OSTI ID:5211575

Shank, B; Andreeff, M; Li, D

Cell survival kinetics in both peripheral blood and in bone marrow have been studied over the time course of hyperfractionated total body irradiation (TBI) for bone marrow transplantation. Our unique TBI regimen allows the study of the in vivo radiation effect uncomplicated by prior cyclophosphamide, since this agent is given after TBI in our cytoreduction scheme. Peripheral blood cell concentrations were monitored with conventional laboratory cell counts and differentials. Absolute bone marrow cell concentrations were monitored by measuring cell concentrations in an aspirate sample and correcting for dilution with blood by a cell cycle kinetic method using cytofluorometry. For lymphocytes in peripheral blood in patients in remission, the effective D/sub 0/ ranged from 373 rad in 10 children less than or equal to 10 y old, to 536 rad in the four patients between 11 to 17 y old, while n = 1.0 in all groups. There was no trend observed according to age. Granulocytes had a much higher effective D/sub 0/, approximately 1000 rad in vivo. Absolute nucleated cell concentration in marrow dropped slowly initially, due to an increased lymphocyte concentration in marrow during a concurrent drop in lymphocyte concentration in peripheral blood, but eventually fell on the last day of TBI ranging from 7 to 44% of the initial marrow nucleated cell concentration. Marrow myeloid elements, however, dropped continuously throughout the course of TBI.

• Research Organization: Memorial Sloan-Kettering Cancer Center, New York, NY
• OSTI ID: 5211575
• Journal Information: Int. J. Radiat. Oncol., Biol. Phys.; (United States), Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Vol. 9:11; ISSN IOBPD
• Country of Publication: United States
• Language: English

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62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BLOOD COUNT
BODY FLUIDS
BONE MARROW CELLS
CELL FLOW SYSTEMS
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
FRACTIONATED IRRADIATION
HARD X RADIATION
HEMIC DISEASES
IONIZING RADIATIONS
IRRADIATION
LEUKEMIA
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MEDICINE
NEOPLASMS
NUCLEAR MEDICINE
PATIENTS
POPULATION DYNAMICS
RADIATION EFFECTS
RADIATIONS
RADIOLOGY
RADIOTHERAPY
SIDE EFFECTS
SOMATIC CELLS
THERAPY
TRANSPLANTS
WHOLE-BODY IRRADIATION
X RADIATION