Effect of ozone on photosynthesis in Euglena gracilis Z

Conference · Tue Apr 01 04:00:00 EST 1986 · Plant Physiol., Suppl.; (United States)

OSTI ID:5202169

Chevrier, N; Sarhan, F; Chung, Y S

Euglena cells were used as a plant cell model to study the effects of O/sub 3/ on photosynthesis. Cells were exposed to 1 ..mu..mole min/sup -1/ O/sub 3/ and viability, as estimated by the trypan blue exclusion test, was 80% after a 2 hr exposure period. In vivo /sup 14/CO/sub 2/ fixation was inhibited by 77% after the 2 hr O/sub 3/ exposure as compared to control (air treated). On the other hand, the Hill reaction rate (DCPI reduction) and chlorophyll content were not significantly changed. These results suggest that O/sub 3/ penetrate beyond the chloroplast envelope and affect the content of stroma, possibly by oxidizing essential SH groups of the CO/sub 2/ reduction cycle enzymes without affecting the thylakoid membranes function. After O/sub 3/ exposure, Euglena cells were transferred to a growth chamber under autotrophic conditions and allowed to recuperate. /sup 14/CO/sub 2/ fixation capacity was totally restored after 20 hrs, indicating that the cells were able to repair the damage inflicted to chloroplasts during O/sub 3/ exposure.

• Research Organization: Univ. of Quebec, Montreal
• OSTI ID: 5202169
• Conference Information: Journal Name: Plant Physiol., Suppl.; (United States) Journal Volume: 80:4
• Country of Publication: United States
• Language: English

Related Subjects

560303* -- Chemicals Metabolism & Toxicology-- Plants-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALGAE
BIOLOGICAL EFFECTS
CARBON 14 COMPOUNDS
CARBON DIOXIDE FIXATION
CHEMICAL REACTIONS
EUGLENA
INJURIES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MASTIGOPHORA
MICROORGANISMS
OZONE
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PLANTS
SYNTHESIS
TRACER TECHNIQUES
UNICELLULAR ALGAE