Probing conformational changes in proteins in mass spectrometry

Journal Article · Wed Nov 21 04:00:00 EST 1990 · Journal of the American Chemical Society; (United States)

DOI:https://doi.org/10.1021/ja00180a074· OSTI ID:5202098

Chowdhury, S K; Katta, V; Chait, B T ^[1]

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1. Rockefeller Univ., New York, NY (USA)

Mass spectrometry has found wide application for the elucidation of the primary structures of proteins. However, with the exception of topographical studies of membrane-bound proteins, mass spectrometry has not previously been utilized to obtain information concerning in three-dimensional conformation of proteins. In the present communication, the authors describe the first use of mass spectrometry for probing conformational changes in proteins in a manner analogous to that employed in techniques like optical rotary dispersion, circular dichroism, and spectrophotometry.

• OSTI ID: 5202098
• Journal Information: Journal of the American Chemical Society; (United States), Journal Name: Journal of the American Chemical Society; (United States) Vol. 112:24; ISSN 0002-7863; ISSN JACSA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
550200 -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
CONFORMATIONAL CHANGES
CYTOCHROMES
DATA
EXPERIMENTAL DATA
INFORMATION
MASS SPECTROSCOPY
MEASURING METHODS
NUMERICAL DATA
ORGANIC COMPOUNDS
PIGMENTS
PROTEIN STRUCTURE
PROTEINS
SPECTROSCOPY