Characterization of oxidation states of the Fe-protein from Azotobacter vinlandii
Thesis/Dissertation
·
OSTI ID:5193443
Some of the properties of the (4Fe-4S) cluster of the Fe protein from Azotobacter vinlandii nitrogenase (Av2) were studied by two methods; Fe chelation reactions and Fe exchange reactions. In the presence of the chelator, 2,2-dipyridyl, and MgATP, the oxidized (4Fe-4S) cluster decomposes in a biphasic manner. In the initial fast phase, two Fe are removed; the remainder of the iron is chelated in a much slower process. An intermediate of the chelation reaction, 2Fe-Av2, contains 2Fe/protein. Iron and inorganic sulfur determinations, and visible, EPR, and Moessbauer spectal properties indicate that the 2Fe-Av2 intermediate of the chelation reaction contains a 2Fe-2S cluster. The Fe in the oxidized (4Fe-4S) cluster can be exchanged with radiolabeled iron without loss of activity of the protein. In 100 mM NaCl and MgATP, only two of the Fe in the cluster are exchanged. MgADP prevents exchange of Fe into the cluster. The Fe in the reduced (4Fe-4S) cluster is apparently resistant to exchange with radiolabeled iron. No conditions are found in which significant exchange of iron is observed.
- Research Organization:
- Minnesota Univ., Minneapolis (USA)
- OSTI ID:
- 5193443
- Country of Publication:
- United States
- Language:
- English
Similar Records
Kinetic and spectroscopic analysis of the inactivating effects of nitric oxide on the individual components of Azotobacter vinelandii nitrogenase
Nitrite, a new substrate for nitrogenase
Probing the MgATP-Bound Conformation of the Nitrogenase Fe Protein By Solution Small-Angle X-Ray Scattering
Journal Article
·
Mon Mar 23 23:00:00 EST 1992
· Biochemistry; (United States)
·
OSTI ID:5403378
Nitrite, a new substrate for nitrogenase
Journal Article
·
Mon Jan 23 23:00:00 EST 1989
· Biochemistry; (USA)
·
OSTI ID:5512705
Probing the MgATP-Bound Conformation of the Nitrogenase Fe Protein By Solution Small-Angle X-Ray Scattering
Journal Article
·
Thu Apr 30 00:00:00 EDT 2009
· Biochem.46:14058-14066,2007
·
OSTI ID:958652
Related Subjects
550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AZOTOBACTER
BACTERIA
CHELATING AGENTS
ELECTRON SPIN RESONANCE
ELEMENTS
IRON
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAGNETIC RESONANCE
METABOLISM
METALS
MICROORGANISMS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PROTEINS
RADIOISOTOPES
RESONANCE
SULFATES
SULFUR COMPOUNDS
TRACER TECHNIQUES
TRANSITION ELEMENTS
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AZOTOBACTER
BACTERIA
CHELATING AGENTS
ELECTRON SPIN RESONANCE
ELEMENTS
IRON
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAGNETIC RESONANCE
METABOLISM
METALS
MICROORGANISMS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PROTEINS
RADIOISOTOPES
RESONANCE
SULFATES
SULFUR COMPOUNDS
TRACER TECHNIQUES
TRANSITION ELEMENTS