Serum factor induces selective increase in Na-channel expression in cultured skeletal muscle

Journal Article · Mon Jul 01 04:00:00 EDT 1991 · Journal of Cellular Physiology; (United States)

DOI:https://doi.org/10.1002/jcp.1041480106· OSTI ID:5191958

Brodie, C; Sampson, S R ^[1]

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1. Bar-Ilan University, Ramat-Gan (Israel)

The authors have examined effects of horse serum (HS) and various fractions (1 million-1M, 300K, 100K, and 30K nominal molecular weight limit) obtained by ultrafiltration on expression of TTX-sensitive Na-channels and on activities of the Na-K pump and glucose transport systems in cultured myotubes obtained from 1-2-day-old neonatal rat pups. Five-day-old cells were transferred to serum-free medium with no hormone or growth factor supplements (DMEM) for 24 hr and then treated with the various serum fractions for 48 hr. Measurements were made of specific (3H)-saxitoxin (STX) binding, action potential properties, 86Rb-uptake and 2-deoxyglucose (2-DG) uptake. HS significantly increased all parameters compared to DMEM (increases in STX-binding, 69%; Rb-uptake, 65%; 2-DG uptake, 93%). Results of treatment with the separate fractions showed that the 300K fraction caused a significantly greater increase in STX-binding than either HS or the other fractions. In contrast, the increases in Rb and 2-DG uptakes induced by the different fractions were not different from that obtained with HS. They conclude that serum contains a factor that selectively increases expression of TTX-sensitive Na-channels in skeletal muscle.

• OSTI ID: 5191958
• Journal Information: Journal of Cellular Physiology; (United States), Journal Name: Journal of Cellular Physiology; (United States) Vol. 148:1; ISSN 0021-9541; ISSN JCLLA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ALKALI METALS
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CARBOHYDRATES
CELL CULTURES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
GLUCOSE
GROWTH FACTORS
HEXOSES
HYDROGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MATERIALS
MEMBRANE TRANSPORT
METALS
MINUTES LIVING RADIOISOTOPES
MITOGENS
MOLECULAR WEIGHT
MONOSACCHARIDES
MUSCLES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
RODENTS
RUBIDIUM 86
RUBIDIUM ISOTOP
SACCHARIDES
SODIUM
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES