Rapid, semimicro method for determination of polycyclic aromatic hydrocarbons in shellfish by automated gel permeation/liquid chromatography
A simple, rapid, easily automated method is described for the determination of polycyclic aromatic hydrocarbons (PAHs) in shellfish such as American lobster (Homarus americanus) and blue mussel (Mytilus edulis). PAHs are extracted from small amounts (1-8 g) of tissue by saponification in 1N ethanolic potassium hydroxide followed by partitioning into 2,2,4-trimethylpentane. This solution is evaporated just to dryness by rotary evaporation and the residue is dissolved in cyclohexane-dichloromethane (1 + 1) for gel permeation chromatography (GPC) on Bio-Beads SX-3. The GPC procedure is ideal as a screening, method in the range 25-18,000 ng PAHs/g tissue. Measurements are required, the appropriate GPC fraction is collected and PAHs are separated by reverse phase liquid chromatography (LC) with fluorometric detection. Individual PAHs at concentrations as low as 0.25-10 ng/g can be determined. Recoveries of added fluoranthene, pyrene, benz(a)anthracene, chrysene, benzo(e)pyrene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(a)pyrene, dibenz(a,h)anthracene, benzo(ghi)perylene, and indeno(1,2,3-cd)pyrene were quantitative, with relative standard deviations ranging from 0.0 to 16.9%.
- Research Organization:
- Fisheries and Oceans Canada, Halifax, Nova Scotia
- OSTI ID:
- 5190105
- Journal Information:
- J. Assoc. Off. Anal. Chem.; (United States), Journal Name: J. Assoc. Off. Anal. Chem.; (United States) Vol. 69:3; ISSN JANCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
400102* -- Chemical & Spectral Procedures
ANIMALS
AQUATIC ORGANISMS
AROMATICS
ARTHROPODS
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
CHROMATOGRAPHY
CRUSTACEANS
DATA
DECAPODS
DECOMPOSITION
EMISSION SPECTROSCOPY
EXPERIMENTAL DATA
FLUORESCENCE SPECTROSCOPY
GEL PERMEATION CHROMATOGRAPHY
HYDROCARBONS
HYDROLYSIS
INFORMATION
INVERTEBRATES
LIQUID COLUMN CHROMATOGRAPHY
LOBSTERS
LYSIS
MOLLUSCS
MUSSELS
NUMERICAL DATA
ORGANIC COMPOUNDS
POLYCYCLIC AROMATIC HYDROCARBONS
QUANTITATIVE CHEMICAL ANALYSIS
SAPONIFICATION
SEPARATION PROCESSES
SOLVOLYSIS
SPECTROSCOPY