Reconstitution of LHC phosphorylation by a protein kinase isolated from spinach thylakoids
Conference
·
OSTI ID:5189351
Protein kinase activity is responsible for phosphorylating the (LHC) light-harvesting chlorophyll a/b protein complex of photosystem II, leading to its migration in the thylakoid membrane, the fractional redistribution of excitation energy between photosystems II and I, and the phenomenon of state transition. Previous work from this laboratory described the purification to homogeneity of a thylakoid protein kinase which catalyzes the phosphorylation of isolated LHC at 1-10% of a rate estimated for this enzyme and substrate when resident together in the thylakoid membrane. In this communication, we report rates of LHC phosphorylation that are close to physiological, in a system comprised of isolated purified protein kinase (LHCK) and native LHC. 9 refs., 1 fig., 2 tabs.
- Research Organization:
- Brookhaven National Lab., Upton, NY (USA)
- DOE Contract Number:
- AC02-76CH00016
- OSTI ID:
- 5189351
- Report Number(s):
- BNL-38632; CONF-860808-3; ON: DE87000513
- Country of Publication:
- United States
- Language:
- English
Similar Records
Purification and characterization of a thylakoid protein kinase
Partial purification of a spinach thylakoid protein kinase that can phosphorylate light-harvesting chlorophyll a/b proteins
Protein kinase that phosphorylates light-harvesting complex is autophosphorylated and is associated with photosystem II
Conference
·
Tue Dec 31 23:00:00 EST 1985
·
OSTI ID:5206232
Partial purification of a spinach thylakoid protein kinase that can phosphorylate light-harvesting chlorophyll a/b proteins
Book
·
Mon Dec 31 23:00:00 EST 1984
·
OSTI ID:5882351
Protein kinase that phosphorylates light-harvesting complex is autophosphorylated and is associated with photosystem II
Journal Article
·
Tue Oct 06 00:00:00 EDT 1987
· Biochemistry; (United States)
·
OSTI ID:5515288
Related Subjects
14 SOLAR ENERGY
140505* -- Solar Energy Conversion-- Photochemical
Photobiological
& Thermochemical Conversion-- (1980-)
550201 -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL REACTIONS
CHLOROPHYLL-BINDING PROTEINS
DAYS LIVING RADIOISOTOPES
ELECTROPHORESIS
ENZYMES
FRACTIONATION
ISOTOPES
LIGHT NUCLEI
MEMBRANES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PHOTOSYNTHETIC MEMBRANES
PHOTOSYNTHETIC REACTION CENTERS
PROTEINS
RADIOISOTOPES
SEPARATION PROCESSES
TRANSFERASES
140505* -- Solar Energy Conversion-- Photochemical
Photobiological
& Thermochemical Conversion-- (1980-)
550201 -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL REACTIONS
CHLOROPHYLL-BINDING PROTEINS
DAYS LIVING RADIOISOTOPES
ELECTROPHORESIS
ENZYMES
FRACTIONATION
ISOTOPES
LIGHT NUCLEI
MEMBRANES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PHOTOSYNTHETIC MEMBRANES
PHOTOSYNTHETIC REACTION CENTERS
PROTEINS
RADIOISOTOPES
SEPARATION PROCESSES
TRANSFERASES