Neomycin resistance as a selectable marker in Methanococcus maripaludis
Journal Article
·
· Applied and Environmental Microbiology
OSTI ID:518247
- Univ. of Washington, Seattle, WA (United States)
- Univ. of Georgia, Athens, GA (United States)
The authors cloned the aminoglycoside phosphotransferase genes APH3{prime}I and APH3{prime}II between the Methanococcus voltae methyl reductase promoter and terminator in a plasmid containing a fragment of Methanococcus maripaludis chromosomal DNA. The resulting plasmids encoding neomycin resistance transformed M. maripaludis at frequencies similar to those observed for pKAS102 encoding puromycin resistance. The antibiotic geneticin was not inhibitory to M. maripaludis. 22 refs., 3 figs., 3 tabs.
- DOE Contract Number:
- FG05-94ER20158
- OSTI ID:
- 518247
- Journal Information:
- Applied and Environmental Microbiology, Vol. 62, Issue 11; Other Information: PBD: Nov 1996
- Country of Publication:
- United States
- Language:
- English
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